Wednesday, 9 July 201409:00 | Introductions | 09:30 | Lecture: Introduction to drug discovery and the design and development of biochemical assays for drug discovery purposes - what can be achieved and learnings from past successes and failures, screening jargon and terms. | 10:00 | Case Study: Biochemical Assay Development and Screening. | 10:30 | Break | 10:45 | Lecture: Reagent characterisation and selection of assays which will ensure translation of Hits between formats. | 11:15 | Overview of practical work for Day 1 and creation of groups for practical work: 1. General concepts for biochemical assays exemplified using kinase, histone deacetylase and protease enzymes. 2. IC50 determination for inhibitor, signal stability, choice of liquid handling and Z' calculation. 3. Application of biochemical assays in drug discovery. | 12:15 | Talk From Vendor (Optional) | 12:30 | Lunch | 13:30 | Experimental Work | 17:00 | Group Dinner |
Thursday, 10 July 201409:00 | Discuss Results from Day 1 | 10:00 | Lecture: Data Analysis and Reduction - Going Beyond the Z'. Discuss methods for analysing in-vitro biological assays data including false positive/negative rates, dose-response curve fitting and correlations. | 11:00 | Break | 11:15 | Overview of Practical Work for Day 2: 1. Screening of biochemical assays against a small molecule library (proof-ofconcept screen). | 12:15 | Lunch | 13:15 | Talk From Vendor (Optional) | 13:30 | Experimental Work | 18:00 | Free Evening |
Friday, 11 July 201409:00 | Discuss Results from Day 2 | 10:00 | Case Study: Analysis of High Throughput Screening Assay Data. | 11:00 | Talk From Vendor (Optional) | 11:15 | Break | 11:30 | Lecture: Integrating your research program, design of project critical paths which integrate in-vitro, in-vivo and in-silico elements. | 12:00 | Lunch | 13:00 | Each team to compare results and identify learnings from practical course, presentations from each team and wrap up. |
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