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SELECTBIO Conferences Point-of-Care, Biosensors & Mobile Diagnostics Europe 2019

Point-of-Care, Biosensors & Mobile Diagnostics Europe 2019 Agenda



Co-Located Conference Agendas

Lab-on-a-Chip & Microfluidics Europe 2019 | Point-of-Care, Biosensors & Mobile Diagnostics Europe 2019 | Organ-on-a-Chip & Tissue-on-a-Chip Europe 2019 | 

Print Agenda

Tuesday, 18 June 2019

07:45

Conference Registration, Materials Pick-Up, Morning Coffee and Pastries

08:45

Nancy AllbrittonConference Chair

Chairperson's Welcome and Introduction to the Conference
Nancy Allbritton, Kenan Professor of Chemistry and Biomedical Engineering and Chair of the Joint Department of Biomedical Engineering, University of North Carolina and North Carolina State University, United States of America


Session Title: Opening Plenary Session

09:00

Holger SchmidtKeynote Presentation

Optofluidic Labs-on-Chip For Single Molecule Analysis
Holger Schmidt, Narinder Kapany Professor of Electrical Engineering, University of California-Santa Cruz, United States of America

Lab-on-chip devices have long held the promise of providing a convenient and rapid way to analyze small amounts of biological samples. However, when pushed to the ultimate limit of single molecule sensitivity, the detection mechanism is often based on off-chip elements. I will discuss a chip-scale platform that offers both integrated optical and electrical single molecule analysis. Optical integration is achieved by using liquid-core waveguides interfaced with traditional photonic elements to implement advanced functionalities. Examples include multiplex detection of single viruses, simultaneous detection of proteins and nucleic acid biomarkers, and front-to-back sample handling and single DNA detection on a single chip. Electrical single molecule analysis is achieved by integration of solid-state nanopores. Novel nanopore detection capabilities such as feedback-controlled delivery of single molecules to a fluidic channel are demonstrated. The combination of both optical and electrical detection modalities results in a novel, high throughput platform for single molecule analysis.

09:30

Elisabeth VerpoorteKeynote Presentation

Organs-on-Chips: Analytical Tools and Objects for Analysis
Elisabeth Verpoorte, Professor of Analytical Chemistry and Pharmaceutical Analysis, University of Groningen, Netherlands

Organs-on-chips (OoC) can be viewed as biological processing units, meant to replicate on a smaller scale the cell architecture and functioning of the organs they represent. OoC may be used to probe and thus better understand the mechanisms by which organs function. Mostly, though, they will be used as in vitro processing tools, to yield insight as to the fate of compounds like drugs or foodstuffs in vivo. Either way, merging analytical detection technologies with microfluidics and cell culture is key to the development of these systems for advanced in vitro analysis. Several examples of analytical approaches for monitoring liver slice behavior stemming from our labs will be presented in this presentation.

10:00

Thomas LaurellKeynote Presentation

New Opportunities in Acoustofluidic Processing of Liquid Biopsies
Thomas Laurell, Professor, Lund University, Sweden

10:30

Morning Coffee, Pastries and Networking in the Exhibit Hall

11:15

Steve SoperKeynote Presentation

Nanosensor Chips for the Single-Molecule Sequencing of DNA and RNA
Steve Soper, Foundation Distinguished Professor, Director, Center of BioModular Multi-scale System for Precision Medicine, The University of Kansas, Adjunct Professor, Ulsan National Institute of Science & Technology, United States of America

We are generating a single-molecule DNA/RNA sequencing platform that can acquire sequencing information with high accuracy (>99%) at unprecedented throughputs (106 nt/s). The technology employs a fluidic chip populated with nanosensors that read the identity of individual mononucleotides from their characteristic flight-time through a 2-dimensional (2D) nanochannel (~50 nm in width and depth; >10 µm in length) and their current transient amplitudes. The nanosensors are fabricated in a thermoplastic via nanoimprint lithography (NIL). The mononucleotides are generated from an intact DNA fragment using a highly processive exonuclease, which is covalently anchored to a plastic support (500 nm in diameter) contained within a bioreactor that sequentially feeds mononucleotides into a 2D nanochannel. The identity of the mononucleotides is deduced from a molecular-dependent flight-time through the 2D nanochannel that is related to the electrophoretic mobility of that molecule. The flight time is read in a label-less fashion by measuring current transients (i.e., resistive pulse sensing) induced by a single mononucleotide when it travels through a constriction possessing molecular dimensions (<10 nm in diameter) and poised at the input/output ends of the flight tube. In this presentation, our efforts in building these nanosensors using NIL in thermoplastics will be discussed. We will also talk about the detection of single molecules using NIL-produced nanopores. Also, surface modifications of plastics for the immobilization of biologics, such as exonucleases, will be discussed and their enzymatic performance when surface immobilized. Finally, information on the manipulation of single DNA molecules using nanofluidic circuits that uses nano-scale features to shape electric fields will be presented.

11:45

Nancy AllbrittonKeynote Presentation

Intestine on a Chip for Basic Biology and Patient-Specific Medicine
Nancy Allbritton, Kenan Professor of Chemistry and Biomedical Engineering and Chair of the Joint Department of Biomedical Engineering, University of North Carolina and North Carolina State University, United States of America

The ability to monitor and control the environment at the cellular and tissue level is one of the most promising applications for microengineered systems. Advances over the past decade in our ability to isolate and culture stem cells when combined with microengineering make it possible to conceive of physiologically functional systems that reconstitute whole organ physiology. These “organ-on-a-chip” platforms enable the establishment of the tissue interfaces necessary for organ function while providing exquisite control of experimental variables and sharing the richness of the intact organism. My group is at the forefront of marrying these advances in stem-cell culture with microengineering to create in vitro tissue mimics with a focus on the intestine. We constructed in vitro epithelium for human and mouse, small and large intestine that bear a stunning physical resemblance to the in vivo intestinal epithelium epithelia displaying arrays of polarized crypts. Most importantly this system recapitulates much of the physiology of the native intestinal epithelium. The system is constructed by developing a self-renewing monolayer of primary cells which is then shaped using microfabrication techniques. The platform enables application of the diverse chemical gradients (growth factors, morphogens, bacterial metabolites, food substances, and gases) thought to exist along the crypt-villus axis.  Application of gradients of microbiota-derived fermentation products across this tissue provided a direct demonstration that these products drive alterations in the size of the crypts’ proliferative and differentiated compartments as predicted to occur in vivo. The system also enables co-culture of anaerobic intestinal bacteria above a physiologic mucus layer. Using this platform, small intestinal biopsies from humans can be used to populate the constructs with cells producing patient-specific tissues for personalized medicine.

12:15

Shoji TakeuchiKeynote Presentation

Emerging Technologies For Biohybrid Machines
Shoji Takeuchi, Professor, Center For International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science, The University of Tokyo, Japan

Engineers have created various kinds of machines such as smart phones, humanoid robots, self-driving cars, etc. However, there are still big hurdles to build a system that demonstrates attractive functions appearing in biological systems, such as single molecule recognition, highly efficient biomolecular production, biocompatibility and self-healing/self-reproducing ability etc…

To achieve this function, we have studied biohybrid machines that harnesses the living system within artificial systems. In this symposium, I would like to talk about a couple of our recent results regarding biohybrid sensors and actuators.

12:45

Martyn BoutelleKeynote Presentation

Title to be Confirmed.
Martyn Boutelle, Professor of Biomedical Sensors Engineering, Vice Chair Department of Bioengineering, Imperial College London, United Kingdom

13:15

Networking Lunch in the Exhibit Hall and Poster Viewing


Session Title: Emerging Trends and Themes in Point-of-Care Diagnostics and Mobile Diagnostics

14:15

Commercialization of Microfluidic Devices for Point-of-Care Applications
Vincent Linder, Founder and President, CDP BioMedical Consulting, Portugal

This presentation will focus on the commercialization challenges specific to microfluidic devices for point-of-care applications.  At an early stage of development, strategies can focus on de-risking analytical and clinical performances and may overlook other critical aspects of the design.  For a successful commercialization effort, it is essential to implement at the onset of the program a comprehensive vision encompassing the patient presenting in a POC setting, the POC user and all the steps needed to obtain an actionable test result.  This presentation will focus on selected commercialization challenges and discuss directions/solutions to overcome them.

14:45

Planar Waveguide Biosensor Technology for Next Generation Point of Care Diagnostics
Reuven Duer, Founder & CEO, Proactive Diagnostics, Inc., United States of America

I’ll be presenting a novel and disruptive detection technology for Point of Care diagnostics.  The presentation includes the underlying physics, implementation solutions and testing data of this unique technology platform.

15:15

Label-Free Biosensing With Impedimetric and Thermal Detection Methods
Patrick Wagner, Professor, Soft-Matter Physics and Biophysics Section, Departement Natuurkunde en Sterrenkunde, Katholieke Universiteit Leuven, Belgium

The first part of this presentation addresses the detection of single-nucleotide polymorphisms in DNA by monitoring the denaturation of double-stranded DNA fragments on solid chips such as synthetic diamond coatings. Denaturation experiments were performed in two different ways: i) Chemically, with NaOH solutions in combination with impedance spectroscopy as readout principle and ii) thermally, using the heat-transfer method HTM. The latter technique measures the thermal interface resistance Rth between the chip and the liquid; the Rth parameter responds sensitively to all molecular changes at the solid-liquid interface.

Using these two methods, I will address a couple of “real life” biosensing applications such as the detection of the allergen Ara h1 (a protein) in peanut butter, the neurotransmitter serotonin in blood, and histamine in fish brine and intestinal fluids. Histamine is a mediator in the irritable bowel syndrome IBS and we are currently working towards a catheter-based diagnostic tool. Within these applications, polymer-based receptors (molecularly imprinted polymers, MIPs) are playing an advantageous role owing to their long-term stability, chemical resilience under adverse conditions, and their regeneration capacity.   

In the final part of the presentation, I will discuss the selective detection and identification of cells (e.g. macrophages, cancer cells) and bacteria using whole-cell receptors. These receptors, surface-imprinted polymers (SIPs), are fabricated by soft lithography with stamping of template cells onto polyurethane layers. Special attention goes to the rebinding mechanism between the imprints and target cells and to ways how to boost selectivity by repetitive exposure. Combining these SIP receptors with HTM readout, relevantly low detection limits are becoming feasible. Especially for agriculture, the food industry and in the context of environmental safety, there is a considerable need for fast, on-site techniques to detect pathogenic microorganisms.

15:45

Afternoon Coffee Break and Networking in the Exhibit Hall

16:30

Rapid Diagnosis of Breast Cancer: Innovative Approaches with a Focus on Low Resource Setting
Jane Brock, Chief of Breast Pathology, Brigham and Women’s Hospital, Harvard Medical School, United States of America

Breast Cancer care includes prevention, early detection, diagnostics and therapeutics. Therapeutic decisions are made based on traditional prognostic factors including tumor size, lymph node status, and factors obtained from pathological assessment including tumor grade, immunohistochemical profile of Estrogen and Progesterone Receptor (ER and PR) and Her2/neu gene amplification status. Point of care technology is not currently used in this routine pathological assessment, but there are new opportunities to expedite and facilitate diagnosis, primarily driven by the need to provide breast cancer diagnoses in low-resource settings to the tens of thousands of women who develop breast cancer worldwide. This presentation will discuss alternative methods of tissue biopsy, handling, and imaging and prognostic marker evaluation that can obviate the need for expensive traditional processing equipment and microscopes, and can allow for more rapid cancer diagnosis and biomarker evaluation compared with current traditional methods.

17:00

Paper-based Analytical Devices For the Diagnosis and Monitoring of Infectious Diseases at the Point of Care
Jean-Manuel Segura, Professor, University of Applied Sciences and Arts Western Switzerland Valais, Switzerland

The diagnosis and treatment of infectious diseases require many in vitro diagnostic (IVD) tests to guarantee an accurate and efficient intervention. First, testing of generic inflammation markers such as CRP enable a rapid determination of the origin of the infection, either bacterial or viral. Then detailed analysis of genetic material or of specific biomarkers enables identification of the pathogen in order to select the most appropriate treatment. Finally, monitoring of the administrated therapeutic drugs in the blood allows for personalizing the dose in order to optimize efficacy while avoiding side effects. Performing these different tests at the point of care provides many benefits, in particular a more rapid intervention.

Paper offers many advantages as a material to produce IVD tests as it is cheap, amenable for mass manufacturing, easy to dispose after performing the test and it provides many functionalities for designing a test such as capillary-driven motion of liquids. Two main types of paper-based analytical tests exist with the lateral-flow assays, ideal to perform immunoassays, and the microfluidic paper-analytical devices, microPAD, ideal to perform enzymatic assays.

I will present our most recent results in developing various paper-based IVD tests for the diagnosis and treatment of infectious diseases at the point of care. A particular emphasis will be put on a microPAD test for the monitoring of therapeutic antibiotics in the blood.

17:30

Arben MerkoçiKeynote Presentation

Nanobiosensors for Diagnostics Applications
Arben Merkoçi, ICREA Professor and Director of the Nanobioelectronics & Biosensors Group, Institut Català de Nanociencia i Nanotecnologia (ICN2), Barcelona Institute of Science and Technology (BIST), Spain

There is a high demand to develop innovative and cost effective devices with interest for health care beside environment diagnostics, safety and security applications. The development of such devices is strongly related to new materials and technologies being nanomaterials and nanotechnology of special role. We study how new nanomaterials such as nanoparticles, graphene, nano/micromotors can be integrated in simple sensors thanks to their advantageous properties. Beside plastic platforms physical, chemical and mechanical properties of cellulose in both micro and nanofiber-based networks combined with their abundance in nature or easy to prepare and control procedures are making these materials of great interest while looking for cost-efficient and green alternatives for device production technologies. Both paper and nanopaper-based biosensors are emerging as a new class of devices with the objective to fulfil the “World Health Organization” requisites to be ASSURED: affordable, sensitive, specific, user-friendly, rapid and robust, equipment free and deliverable to end-users. How to design simple paper-based biosensor architectures? How to tune their analytical performance upon demand? How one can couple nanomaterials such as metallic nanoparticles, quantum dots and even graphene with paper and what is the benefit?   How we can make these devices more robust, sensitive and with multiplexing capabilities? Can we bring these low cost and efficient devices to places with low resources, extreme conditions or even at our homes? Which are the perspectives to link these simple platforms and detection technologies with mobile communication? I will try to give responses to these questions through various interesting applications related to protein, DNA and even contaminants detection all of extreme importance for diagnostics, environment control, safety and security.

18:15

Networking Reception with Beer and Wine in the Exhibit Hall -- Meet Colleagues and Network with Exhibitors

19:15

Close of Day 1 of the Conference

Wednesday, 19 June 2019

08:00

Morning Coffee, Pastries and Networking in the Exhibit Hall


Session Title: Technologies in Biosensors and Point-of-Care, Point-of-Need Diagnostics

09:00

Jonathan CooperKeynote Presentation

Origami Paper Folding Enabling DNA Diagnostics in Under-served Rural Communities East Africa
Jonathan Cooper, Wolfson Professor and University Vice Principal, Glasgow University, United Kingdom

We have devised an origami-inspired paper device as a low-cost, rapid, and easy-to-use field test to help combat the spread of infectious diseases, like malaria. Although healthcare workers utilize various accepted methods, many standard tests have proven unreliable and impracticable, especially in remote, rural communities that lack refrigeration and laboratory equipment. To address the growing need for better tests, we have developed a paper, origami-inspired field device that provides fast, high-quality, species-specific malaria diagnostics that compare favorably to PCR laboratory assays, the current gold standard. Requiring just a wax printer and hot plate, the design draws on origami folding techniques to create a paper device which prepares blood samples for a field-friendly amplification technique known as loop-mediated isothermal amplification, or LAMP. The device is further augmented with microfluidic lateral flow LAMP amplification and a simple detection platform that can diagnose malaria species from a finger-prick blood sample. As a demonstration, we present a double-blind, first-in-human trial with rural primary schools in Mayuge and Apac Districts in Uganda that show the device outperforms other established techniques, detecting malaria in 98% of infected participants. Finally we also show that the techniques can be used in a variety of environmental and veterinary applications, with sampling from faeces, sperm and water in studies in India and China.

09:45

The Legal and Regulatory Landscape of Diagnostics Devices
Erik Vollebregt, Partner, Axon Lawyers, Netherlands

The legal and regulatory landscape of diagnostics devices in the EU is in full flux as a result of the In Vitro Diagnostics Regulation, the General Data Protection regulation and other developments like the Brexit. This presentation will address where the rules are at this moment and what this means for the diagnostics industry.

10:15

Smart Thermometers: Screening for Biomarkers Using Molecularly Imprinted Polymers (MIPs) Combined with Thermal Detection
Marloes Peeters, Lecturer, Manchester Metropolitan University, United Kingdom

Molecularly Imprinted Polymers (MIPs) are synthetic antibody mimics: similar to antibodies they possess high affinity for a chosen template molecule. However, they have distinct advantages over their natural counterparts such as low-cost, superior chemical and thermal stability, and straightforward production process. In this contribution, we will discuss the use of nanoMIPs that are synthesized via a solid-phase approach. The high affinity nanoparticles prepared by this technique are water-soluble, meaning it is simple to directly functionalize them onto thermocouples via dipcoating. These functionalized thermocouples were subsequently inserted into a home-made heat-transfer device that measures the temperature of the liquid in a flow cell. It was shown that binding of the target to the MIP layer increased the resistance at the solid-liquid interface, leading to a lower temperature being recorded by the functionalized thermocouple. With this method, it was possible to measure various compounds, ranging from small targets such as the antibiotic vancomycin to larger biomarkers such as troponin, in the low nanomolar regime in buffered solutions. The sensitivity was significantly enhanced by using standard EDC coupling to covalently attach the nanoparticles to the surface, allowing to control the surface architecture. Finally, the design of the current flow cell was adjusted to incorporate four thermocouples, demonstrating the first multiplex format of this sensor platform. In this presentation, a case study on the detection of cardiac biomarkers, including troponin, will be discussed.

10:45

Morning Coffee Break and Networking in the Exhibit Hall

11:30

Ultrafast and Sensitive qPCR-based Detection of Group B Streptococcus (GBS) in Patient Samples in a Matter of Minutes
Maarten Fauvart, R&D Team Leader / Senior Scientist Life Science Technologies, IMEC, Belgium

We present a mass-producible silicon chip-based system for qPCR detection of group B streptococci in five minutes. The analytical performance of the ultrafast qPCR system matches that of the benchtop assay based on a comparison using over 100 patient samples.

12:00

Eloisa Lopez-CalleKeynote Presentation

A Microfluidic Immunoassay Format For the Determination of Cardiac Troponin T at the Point-of-Care
Eloisa Lopez-Calle, Head Assay Formats, Roche Diagnostics GmbH, Germany

A cardiac troponin T assay was developed in a microfluidic immunoassay format for the determination of the analyte with lab-like performance and meeting current clinical guidelines. The assay was completely run in a ready-to-use disposable with fully integrated reagents required for the analysis within less than 12 minutes. After sample application, 30µL of whole blood, the assay was automatically processed in the instrument, firstly by generating plasma via centrifugation, followed by incubation of a defined volume of the generated plasma with the immunoreagents. After washing, the fluorescence in the signal zone of the membrane was measured for the quantitation of cardiac troponin T.

12:45

Networking Lunch in the Exhibit Hall and Poster Viewing

14:00

John BrennanKeynote Presentation

Integrating Aptamer Technology with Paper-Based Point-of-Care Devices for Biomedical Monitoring
John Brennan, Professor and Director, Biointerfaces Institute, McMaster University, Canada

DNA aptamers and DNA enzymes (denoted as functional nucleic acids or FNA) are an emerging platform for development of point-of-care (POC) diagnostic devices.  In this presentation, I will first focus on the development of new aptamers and DNA enzymes for a range of key biomarkers and their integration into colorimetric and fluorimetric assays for a variety of targets, mainly in the area of infectious disease.  Methods to couple target-binding to FNAs to the production of a DNA strand as an output will then be described.  The use the output DNA to directly initiate color production or produce isothermal amplification (ITA), will then be outlined.  Finally, the integration of the FNA assays into flow-based paper devices will be described as platform for a range of new POC devices that allow facile detection of clinical analytes.  Examples will be provided outlining paper-based devices for ultra-sensitive detection of E. coli, C. difficile, MRSA and H. pylori.

14:45

Emmanuel DelamarcheKeynote Presentation

Precise, Intelligent, Mobile and Secure POC Diagnostics: How Can Tech Help?
Emmanuel Delamarche, Manager Precision Diagnostics, IBM Research - Zurich, Switzerland

Diagnostics are ubiquitous in healthcare because they support prevention, diagnosis and treatment of diseases. Specifically, point-of-care diagnostics are particularly attractive for identifying diseases near patients, quickly, and in many settings and scenarios. One of our contribution to the field of microfluidics is the development of capillary-driven microfluidic chips for highly miniaturized immunoassays. In this presentation, I will review how to program capillary flow and encode specific functions to form microfluidic elements that can easily be assembled into self-powered devices for immunoassays, reaching unprecedented levels of precision for manipulating samples and reagents. This technology can also be augmented using peripherals and smartphones for flow control and monitoring with sub-nanoliter precision. Finally, counterfeiting of point-of-care diagnostics is an issue, with sometimes dramatic consequences. Using capillary phenomena, we devised a method for producing in chips a complex signal with a “time domain” for authentication of devices. All together, capillary-driven elements can bring extremely high control for manipulating sub-microliter volumes of samples and picogram quantities of reagents and may therefore extend the performances of microfluidic devices for point-of-care diagnostics to a next level of precision.

15:30

Afternoon Coffee Break and Networking in the Exhibit Hall

16:00

Poster Awards

16:15

Enzymatic Biosensor to Support the Diagnosis of Adrenal Gland Tumors
Michael Schöning, Professor and Director, Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, Germany

Primary aldosteronism (PA) is a disorder in which aldosterone production is inappropriately high and causes hypertension. The clinical guidelines advocate using adrenal vein sampling (AVS) to diagnose PA. However, since AVS is a demanding procedure in which correct cannulation of adrenal veins is challenging, it is used only in a few centers worldwide. To facilitate this medical examination, the detection of adrenaline during AVS could be used as an indicator for the correct position of the catheter, since the adrenaline concentration in adrenal blood (100 nM) is about 100 times higher in comparison to peripheral blood (1 nM).

Two different biosensor approaches for the detection of adrenaline will be presented, which are based on the substrate recycling principle. The first variant is based on an amperometric bi-enzyme biosensor with a genetically modified laccase and a glucose dehydrogenase (GDH). The second example represents a chip-based biosensor with the enzyme PQQ-GDH. Both sensor types have been optimized under laboratory conditions and successfully applied for first measurements in real blood samples, to define an appropriate catheter position.

16:45

The SnapChip™ for a Precise Chip-to-Chip Transfer of Nanodroplets
Sebastien Bergeron, Founder and CEO, Parallex BioAssays Inc., Canada

Multiplexed sandwich immunoassays are a powerful technique to measure multiple protein concentrations simultaneously. However, multiplexed assays are prone to cross reactivity effects and can generate false positive signals due to a lack of antibody specificity and selectivity. This is a known weakness of multiplexed assays that is mitigated by months of assay optimization and validation.

Parallex BioAssays replaced the “detection soup” of antibodies by an array of nanodroplets and offers a new CHIP-to-CHIP approach to deliver the detection antibodies precisely to their cognate spots of capture antibodies. As a result, a cross-reaction free chip consisting of an assembly of parallelized assays, physically isolated from each other. The advantages include the possibility to mix-and-match assays and create unique combinations, such as isoforms and phospho-specific antibodies side-by-side. This makes our approach to cell signaling studies (e.g. Akt activation pathway) a rapid and easy alternative to performing dozens of western blots.

The SnapChip™ represents a new format of liquid handling platforms. Beyond exclusive immunoassays, it creates the opportunity to miniaturize and multiplex enzyme activity and cell-based assays in nano-compartments. Current and future applications will be discussed.

17:15

Title to be Confirmed.
Karolien De Wael, Professor, Antwerp University, Belgium

17:45

Close of Day 2 of the Conference


Add to Calendar ▼2019-06-18 00:00:002019-06-19 00:00:00Europe/LondonPoint-of-Care, Biosensors and Mobile Diagnostics Europe 2019Point-of-Care, Biosensors and Mobile Diagnostics Europe 2019 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com