Co-Located Conference AgendasCirculating Biomarkers and Extracellular Vesicles Europe 2024 | Lab-on-a-Chip and Microfluidics Europe 2024 | Organoids and Spheroids Europe 2024 | Point-of-Care, Biosensors and Rapid Dx Europe 2024 |
Monday, 24 June 202408:00 | Please View Conference Programming Details in the:
LOACEU2024 Track Morning Plenary Session -- Coolsingel Room ORGANOIDS Track Afternoon Session -- Coolsingel Room
EV Track Afternoon Session -- Conrad Room |
Tuesday, 25 June 202408:00 | Please View Conference Morning Programming Details in the:
LOACEU2024 -- Coolsingel Room
Organoids & Spheroids -- Conrad Room
| 12:30 | Networking Buffet Lunch -- Network with Exhibitors and View Posters | | Session Title: Emerging Technologies and Themes in the Point-of-Care and Rapid Diagnostics Field |
| | | Session Venue: Coolsingel Room |
| | | Session Chairperson: Professor Dr. Nicole Pamme |
| | 13:30 | The Power of Scalable POC-Manufacturing in Challenging Times (PPWR, REACH, EU-Legislation) Wouter Geurts, Business Development Manager - FILMS , TEKNI-PLEX , Belgium
In this brief presentation we’ll touch on the topics of IVD regulation, REACH(PFAS) and PPWR, the Packaging & Packaging Waste Regulation (*). We’ll also give an update on material innovation using COC and COP and share an insight on advanced technology that has its roots in the German automotive industry that can revolutionize manufacturing in microfluidics.
(*) just before EU election, so the topic will be super hot. | 14:00 | | Keynote Presentation Microfluidic Devices for On-Site Chemical Sensing Nicole Pamme, Professor in Analytical Chemistry, Stockholm University, Sweden
Our research centers on the study of microfluidic lab-on-a-chip devices applied to environmental analysis, biomedical research and the synthesis of smart materials. Microfluidic devices offer the possibility for in-the-field and point-of-care analysis provided the devices are portable, require only minimal external instrumentation and little power and are robust. In our group, we are investigating a developing paper microfluidic devices for on-site measurement of water quality markers that are being tested by members of the general public who upload data via a custom-built app for data collection with high spatial and temporal resolution. Furthermore, we develop on-site workflows that enable farmers in resource-limited settings to monitor soil chemistry on their own land. |
| 14:30 | | Keynote Presentation ESA SciSpacE Human Research Programme Ines Antunes, Science Coordinator, European Space Agency (ESA), Netherlands
Presentation of the challenges faced by astronauts during long-duration space missions and how the European Space Agency (ESA) is supporting their health and performance through research on the impact of space conditions on the human body and the development of countermeasures and point of care technologies to investigate changes and mitigate those consequences. The presentation will focus on ESA's SciSpacE team, which conducts research in ground analogues, suborbital platforms, and on the International Space Station (ISS), and how the research conducted in space can benefit terrestrial medicine. The process of ideation, call for proposals, review, selection, and implementation of research projects will also be outlined. The presentation aims to provide insights into how ESA is working to keep astronauts healthy and productive during long-term space missions and how these efforts can also benefit medical science on Earth. |
| 15:00 | Mid-Afternoon Coffee Break and Networking | 15:30 | Best Poster Awards Sponsored by Lab-on-a-Chip Journal, Royal Society of Chemistry (RSC) -- In the Coolsingel Room | 16:00 | Tackling the Global Health Threat Posed by Fungal and Oomycete Diseases Using Lab-on-a-Chip Devices Ayelen Tayagui, Research Fellow, University of Canterbury, New Zealand
Lab-on-a-Chip devices can enhance the study of disease-causing microorganisms and tackle their effects on global health. We describe the design, manufacture and use of devices use to study the forces these organisms use to to infect their hosts. | 16:30 | Towards Continuous Cytokine Monitoring in Organ-based Platforms Maud Linssen, Researcher, Eindhoven University of Technology (TU/E), Netherlands
Investigations on organ-based platforms such as organoids,
organ-on-a-chip, and transplantation organs, require monitoring
strategies in order to optimally control the biological systems.
However, there is a lack of tools to continuously measure specific
low-concentration biomarkers with minimal perturbation. Biosensing by
Particle Motion (BPM) is a sensing method with single-molecule
resolution that has been specifically designed to enable the continuous
monitoring of biomolecules at low concentrations, such as nucleic acids,
metabolites, proteins and hormones. The method relies on tracking the
motion of individual biofunctionalized particles (1 µm in diameter) that
interact with a biofunctionalized substrate. The particles switch
between bound and unbound states due to reversible single-molecule
interactions, dependent on the presence of analyte molecules. Recently,
sampling by microdialysis has been investigated [3]. In this paper we
present the development of a BPM sensor to measure cytokines,
exemplified with the detection of Interleukin-6 and TNF-a. We will
present a study of molecular binders and coupling strategies, focusing
on sensitivity and reversibility of the sensor. Furthermore,
measurements with microdialysis will be shown. Finally, we will discuss
the prospects of using BPM and microdialysis for the continuous
monitoring of low-concentration biomarkers in organ-based platforms. | 17:00 | ElectroMed – A Programmable Peptide/Protein Screening Platform for Immunology Cesar Pascual García, Lead Research Scientist, Luxembourg Institute of Science and Technology
Immunology has advanced significantly in recent years thanks to a better knowledge of molecular protein recognition, which has allowed for the creation of innovative treatment options such as genetic vaccines and cell therapies. However, there is a need to accelerate the process of epitope recognition by proteins in order to generate personalized therapies. ElectroMed has been a project with six European partners in which we created a concept for a programmable peptide screening platform based on an array of Immuno-FET sensors with programmable peptide receptors synthesized in-situ using electrochemically generated acid.
A proof-of-concept has been demonstrated by controlling the deprotection of acid labile groups, which enables a high purity synthesis of crude peptides. We remove diffusion-limited processes in chemistry by automating the peptide synthesis and using microfluidic flow in-situ synthesis in planar sensors, allowing us to expedite time to results. FET sensors have the potential to provide online quality control of peptide synthesis by detecting the fingerprints of acid labile group protection and deprotection, as well as superior information quality because label-free sensors can provide kinetic information.
In conclusion, our approach has the potential to speed research into peptide protein screening of linear peptide sequences. In this talk we will review our results and provide the context for the applications in immunology. | 17:30 | | Keynote Presentation Title to be Confirmed. Erik Vollebregt, Partner, Axon Lawyers, Netherlands
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| 18:00 | Parallel High-Throughput Single-Cell Printing Platform for Optoporation-Mediated Large Cargo Delivery Gayathri R, PhD Scholar (Student), Indian Institute of Technology (IIT)-Madras, India
Effective intracellular delivery of biomolecules is crucial in analysing and engineering cell functions, for applications in therapeutic development, diagnostics, and drug delivery towards personalised medicine. Traditional bulk cell culture models employed so far for intracellular delivery, overlook cell population heterogeneity, while single-cell patterning offers robust statistical analyses without compromising cellular variability. In this work, a high-throughput single-cell patterning approach using microcontact printing is employed, where a micro-pillar stamp is used to imprint distinct proteins and finally pattern single-cell to small clusters of cells depending on the micro-pillar diameters. This approach is universal for any protein-cell combination and has 97-99 % patterning efficiency. This single-cell patterning was used as a platform for massively parallel optoporation-mediated intracellular delivery of small to very large biomolecules such as PI dye (668 Da), Dextran (3000 MW), SiRNA (20-24 bp), and enzyme (464 kDa). An easy-to-fabricate and simple-to-use 2D array of titanium micro-dish device is used to facilitate near infrared mediated optoporation. which disrupts the cell plasma membrane, allowing biomolecules to enter into cells with high efficiency (~96±2%) and cell viability (~98±1%). Our compact, robust, and simple-to-print approach has potential for single-cell therapy and diagnostics research, providing high specificity and subcellular accuracy in single-cell level biological processes. | 18:30 | Close of Day 2 Conference Programming |
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