Diagnostic Applications of Exosomes Agenda

Registration

Tracking Exosomes In Vivo; Lessons from a Virus
Danijela Koppers-Lalic, Senior Post Doctoral Researcher, Vu University Medical Center, Netherlands

In anticipation on the clinical trend that cancer tissue(s) will become less available for diagnostic purposes in the future we are developing minimally-invasive molecular diagnostic methodologies based on disseminated microvesicles (MVs) in biofluids.

Coffee Break and Networking in Exhibition Hall

Nanoscale Characterization of Exosomes
Shivani Sharma, Associate Director, California NanoSystems Institute, California NanoSystems Institute, UCLA, United States of America

Exosomes possess unique structural, biochemical, and mechanical characteristics. I will discuss Atomic Force Microscopy (AFM) and correlative microscopy based approaches towards nanoscale characterization of these naturally occurring nano-particles.

Lunch Break and Networking in Exhibition Hall

Poster Viewing Session

Exosomes and MicroRNAs: Market Analysis of the Emerging Diagnostics Biomarkers Space
Enal Razvi, Managing Director, Select Biosciences Inc, United States of America

Select Biosciences has been tracking the microRNA research marketplace over the last several years and more recently has been focused on the applications of microRNA signatures as circulating biomarkers for diagnostics and personalized medicine.

Rapid Isolation of Two Distinct Plasma Membrane Microparticle Populations for Biomarker Discovery and Assay
Sai Kiang Lim, Research Director, Institute of Medical Biology, A*STAR, Singapore

This talk will describe a method for rapid Isolation of two distinct plasma membrane vesicles for biomarker discovery and assay.

Coffee Break and Networking in Exhibition Hall

Polarized Secretion of Exosomes by Epithelial Cells in Health and Disease
Aparna Lakkaraju, Assistant Professor, Ophthalmology and Visual Sciences, University of Wisconsin-Madison, United States of America

This talk will focus on the mechanisms by which polarized epithelial cells secrete distinct populations of exosomes apically and basolaterally, and how diseases that affect late endosomes/multivesicular bodies alter the protein composition and kinetics of exosome secretion by epithelia. 

Exosome Signalling in Endothelial Function and Angiogenesis
Bas WM van Balkom, Assistant Professor, University Medical Center Utrecht, Netherlands

Endothelial cell-derived exosomes play a role in the formation and maintenance of blood vessels. In this presentation, the function and diagnostic potential of these exosomes will be discussed.

Delivery on Call: The Role of Exosomes in Neural Cell Communication
Eva-Maria Krämer-Albers, Senior Scientist, Molecular Cell Biology, Mainz University, Germany

The presentation examines exosome release by oligodendrocytes, the myelinating glia cells of the CNS. It highlights the regulation of glial exosome secretion and exosome transfer to recipient cells and discusses the implications in neural cell communication.

Drinks Reception

Tumor Exosomes and Preparing a Premetastatic Niche
Margot Zoeller, Head of Department, Tumour Progression and Immune Defense, University Hospital of Surgery, Germany

Tumor exosomes reach premetastatic organs, where exosome uptake reprogramms stromal cells to facilitate settlement and growth of metastasizing cells. Selectivity of exosome uptake is organized by by exosomal membrane proteins, exosomal miRNA plays a major role in stroma cell reprogramming.

Exosomes as a Novel Diagnostic and Therapeutic Tool for Cancer
Takahiro Ochiya, Professor, Department of Molecular and Cellular Medicine, Tokyo Medical University, Japan

We present our novel approach to modulate the intercellular network between cancer cells and their environmental cells via silencing signals exported by exosome for cancer development. We also mentioned a novel approach for detecting cancer-specific exosomes.

Exosomes and Microvesicles: Bundles of Biomarkers and Messages to Secondary Cells in Cancer
Lorraine O’Driscoll, Professor in Pharmacology, Trinity College Dublin, Ireland

Coffee Break and Networking in Exhibition Hall

Circulating Exosomal Hsp60 as a New Marker of Colon Cancer
Francesco Cappello, Associate Professor of Human Anatomy, University of Palermo, Italy

A set of in vivo experiment that we performed on tissues and blood samples from patients with colorectal cancer indicate that circulating exosomal Hsp60 has potential as useful diagnostic and prognostic marker.

miRNAs in Biofluids. Understanding and Building Robust Clinical Assays to Analyse Exosomal and Cell-Free Circulating microRNAs.
Hazel Pinheiro, Senior Scientist, Exiqon, Denmark

We present the LNA™-based PCR technology designed to enable the robust detection of microRNAs in biofluids, and introduce our quality control and data analysis pipeline that we have developed to secure high quality reliable microRNA profiling data in a range of biofluid samples.

Lunch Break and Networking in Exhibition Hall

Poster Viewing Session

The Complete Exosome Workflow Solution: From Isolation to Identification of the RNA Markers Using the Ion TorrentTM Personal Genome MachineTM
Susan Magdaleno, Senior Manager, Life Technologies, United States of America

The complete exosome workflow solution will be presented, starting from fast and efficient isolation of exosomes from blood and other body fluids to characterization of their RNA content using the Ion TorrentTM Personal Genome MachineTM.

Exosomes in TB Diagnostics
Jeff Schorey, Professor, University of Notre Dame , United States of America

In this presentation I will summarize our work on the composition and function of exosomes released from Mycobacterium tuberculosis-infected macrophages as well as from infected mice and tuberculosis patients.  I will also highlight the potential use of exosomes in tuberculosis diagnostics.

Coffee Break and Networking in Exhibition Hall

Omics-Technologies Applied to the Study of Extracellular Microvesicles to Identify Candidate Non-Invasive Biomarkers for Liver Injury
Juan Falcon-Perez, Principal Investigator, CIC bioGUNE, Spain

Our group have studied extracellular microvesicles released from hepatocytes challenged with different toxins, and by quantitative proteomics a number of non-invasive candidate markers for liver damage has been identified.

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