Abstract

Optical and Electrical Single Molecule Analysis With Nanopore Optofluidic Devices

Holger Schmidt, Narinder Kapany Professor of Electrical Engineering, University of California-Santa Cruz

In recent years, single molecule analysis has gained increasing importance for next-generation medical diagnostics. Among the leading approaches are optical fluorescence detection and electrical analysis of ionic current across nanopores. I will discuss an integrated platform that can combine both approaches in a single chip. Design and applications of these electro-optofluidic chips will be discussed. These include multiplex detection of multiple molecular biomarkers, dual-mode detection of single viruses, and feedback controlled gating of a nanopore for on-demand delivery and selection of biomolecules for chip-based analysis. The advanced level of electrical, optical, and fluidic integration is ideal for point-of-care applications.