Abstract

Analyzing Extracellular Vesicles With The Amnis® ImageStream®X Mk II High Gain Mode

Haley Pugsley, Manager and Senior Scientist, Luminex Corporation

The importance of EVs has become apparent, as they are key mediators of intercellular communication. However, quantifying and characterizing EVs in a reproducible and reliable manner is challenging due to their small size—exosomes range from 30 to 100 nm in diameter. Flow Cytometry is an attractive method for measuring EVs; however, this has been challenging since fl ow cytometers were designed to measure and detect cells. High Gain mode on the Amnis® ImageStream®X Mk II Imaging Flow Cytometer was designed to address the challenges of measuring particles that are much smaller than cells. This presentation demonstrates the improved small particle detection, including EVs, using this new High Gain mode.