Abstract

Extracellular Vesicles: Paving the Way to Automation and Standardization in Precise Isolation and Single-particle Characterization

Stephane Mazlan, Scientific and Business Coordinator, Izon Science

The focus on Izon Science lies in the standardization of extracellular vesicle (EV) isolation and characterization. The addition of automation brings much needed efficiency and precision in this already complex field of research. Used in conjunction with the Automatic Fraction Collector (AFC), our qEV columns allow efficient EV isolation by not only preserving EV morphology but also providing higher EV purity. In terms of EV measurement, the level of detail and certainty that Tunable Resistive Pulse Sensing (TRPS) offers allow for high resolution especially so where size heterogeneity is an aspect. Capable of measuring EVs as small as 40 nm, our current TRPS instrument, The Exoid, provides not only the accuracy and resolution needed in EV measurements, but it also automizes them, contributing to efficiency in data collection. The EV research community is beginning to make advances in developing EVs as a new class of biological therapeutics. As the potential for EV therapeutics grows, so does the need for precise isolation and measurement of these complex nano biological particles – a challenge that Izon strives to achieve.