Abstract

Real-Time Biosensors: Continuous Measurements of Biomolecules in Live Subjects

H Tom Soh, Professor, Stanford University

A biosensor capable of continuously measuring specific molecules in vivo would provide a valuable window into patients’ health status and their response to therapeutics. Unfortunately, continuous, real-time molecular measurement is currently limited to a handful of analytes (i.e. glucose and oxygen) and these sensors cannot be generalized to measure other analytes.  In this talk, we will present a biosensor technology that can be generalized to measure a wide range of biomolecules in living subjects.  To achieve this, we develop synthetic antibodies (aptamers) that change its structure upon binding to its target analyte and produce an electrochemical current or emit light. Our real-time biosensor requires no exogenous reagents and can be readily reconfigured to measure different target analytes by exchanging the aptamer probes in a modular manner. Using our real-time biosensor, we demonstrate the first closed loop feedback control of drug concentration in live animals and discuss potential applications of this technology.  Finally, we will discuss methods for generating the aptamer probes which are at the heart of this biosensor technology.