Abstract

Ease of Developing 40+ Marker Flow Panels

Anita Kant, Field Applications Scientist, Standard BioTools, Inc.

CyTOF technology provides distinct advantages over other single-cell technologies for highly multiplexed analysis of cell phenotype and function. Mass cytometry uses metal conjugated antibodies for flexible and simple panel design and a streamlined workflow to optimize a 40+ marker panel and significantly reduce time from planning to results. In this master class, we will introduce the principles of panel design with our panel design tool and a universal ten-tube titration method to titrate and validate a 40+ marker mass cytometry panel.