Abstract

New Fluorescent Reagents to Enable Highly Multiplexed Cellular Measurements

Daniel Chiu, Professor, University of Washington

Fluorescence based techniques have become an indispensible tool kit in both basic cellular studies and in vitro diagnostics. However, the intrinsic limitations of conventional dyes, such as short Stoke’s shift and low absorptivity, have posed difficulties for advancing highly multiplexed assays. We have developed a new class of fluorescent probes called Pdots, and this talk will highlight their development and performance to enable highly multiplexed fluorescence measurements in diagnostics.