Abstract

Multicolor, High-Resolution Exosome Analysis with the Delaware Flow NanoCytometer

Giacomo Vacca, President & CEO, Kinetic River Corp

The Delaware Flow NanoCytometer® is a particle analyzer that can measure from EVs to cells. It has a resolution of 6 nm, it can detect 28-nm gold, 60-nm polystyrene, and 68-nm liposomes. We have characterized HansaBioMed FLuoEVs (~90 nm) by scattering, membrane stain, expressed EGFP, and PE-conjugated tetraspanins (CD9, CD63, and CD81). We have benchmarked the Delaware against Particle Metrix’s ZetaView NTA, with close agreement on fluorescent fractions. The Delaware is designed for multiparameter analysis, with up to 6 channels of fluorescence and three scattering channels.