Abstract

Step up your EV Characterization with Leprechaun

Alex Shephard, Market Manager, Unchained Labs

Accurately characterizing extracellular vesicles (EVs) can be challenging, even in highly purified cell culture samples. Interference from lipoproteins, cell debris and protein aggregates make it hard to be confident that you're counting the right stuff. Add in complex biofluids, limited sample volumes, and rare EV subpopulations and the task gets even tougher. Leprechaun lightens the load by isolating EVs on its Luni consumable, before measuring particle size, concentration, and analyzing EV phenotype for up to 4 surface or cargo markers simultaneously, from <25 uL of sample. Leprechaun jives with a range of materials, from crude cell culture media to murine cerebral spinal fluid, without the need for sample purification. With sensitivity down to 5x10^5 particles/mL, single particle analysis and the ability to size EVs as small as 35 nm, Leprechaun is ready to help you step up your EV characterization no matter how rare or small.