Abstract

Bioprinting interfaces for 2D and 3D Cell and Tissue models

Mark DeCoster, Associate Professor, Louisiana Tech University

Biologically compatible interfaces are becoming more important for cell and tissue patterning for both diagnostic and tissue modeling purposes. We have been utilizing patterning interfaces for both two dimensional (2D) and three dimensional (3D) cell assemblies for the purposes of understanding cellular interactions of healthy brain cells as well as those from disease (brain tumors). Using the Nano eNablerTM microprinter system (Bioforce Nanosciences), we have generated defined patterns of fluorescently labeled adhesive nanofilm polymer (poly-lysine), that averaged 5-10 microns in the x and y axis and nanometers in thickness (z-axis). These adhesive patterns induced highly differentiated cells using normal brain glia (astrocytes), with less differentiation but faster growth using glioma cells from brain tumors. Both normal and cancer cell glia adhered to the bioprinted patterns, but the response once bound was different, reflecting functions and dysfunctions of the cells used. We have developed a novel, matrix-free method for generation of 3D cell spheroids, and are combining knowledge from bioprinting methods on 2D surfaces to link 3D cellular structures. Understanding the transition between 2D and 3D cell aggregates will require better bioprinting interfaces to control cellular models and tissue engineering with better fidelity.