Abstract

Advanced Centrifugal Microfluidics for Point-of-Care Applications

Roland Zengerle, Professor, University of Freiburg

Centrifugal microfluidics enables efficient miniaturization, integration, parallelization and automation of biochemical assays especially for Point-of-Care applications. This talk will demonstrate fully integrated sample-to-answer genotyping assays from whole blood. The assay is implemented on a LabDisk and is performed within 2 hours on a small and lightweight instrument. Novel and advanced centrifugal unit operations will be discussed such as integrated pneumatic pumping, automated bead handling as well as the merging of droplet microfluidics with centrifugal microfluidics for digital amplification assays. Finally, challenges and solutions for the scale-up of manufacturing the polymer LabDisk will be discussed.