Abstract

Using ncRNAs to identify cancer cell vulnerabilities

Alexander Pertsemlidis, Associate Professor, Dept. of Pediatrics, Cellular & Structural Biology, The University of Texas Health Science Center at San Antonio

In an unbiased and comprehensive approach, we have combined a high-throughput screening platform with a libraries of inhibitors of short and long non-coding RNAs. We use this platform to identify ncRNAs that reduce cell viability in general, and those that specifically sensitize cells to microtubule-targeting agents. Regulatory targets of candidate ncRNAs are identified and the response of cancer cells to perturbations in candidate ncRNA levels are assessed through a combination of in vitro, in silico and in vivo approaches.