Abstract

Droplet-Based Digital PCR Procedures and Optimized NGS to Track Circulating Tumor DNA in Liquid Biopsies: Application to Cancer Patient Follow-Up

Valérie Taly, CNRS Research Director, Group Leader and Professor, Université Paris Cité

Tracking of circulating cell-free nucleic acids in body effluents has been greatly facilitated by recent technological developments including droplet-based digital PCR and optimized NGS. In particular, droplet based digital PCR has allowed to reach unprecedented sensitivity and accuracy for rare sequences detection. Complementarity of these technologies will be presented through several retrospective and prospective studies. Moreover, we will present their application for the detection of circulating tumor DNA and its potential use for cancer patient treatment management.