Abstract

Guided Tissue Assembly Biofabrication of Epithelial Organoid Tubes

Yan Yan Shery Huang, Lecturer, University of Cambridge

Epithelial organoids cultured in appropriate 3D conditions typically develop a microscopic, cystic structure lined with a polarized epithelium. Despite their great potential in research and therapy, epithelial organoids grow in heterogeneous sizes, and are too small to display physiologically relevant performance and applications. Here, we show guided assembly of mouse tracheal basal stem cell organoids towards geometrically-defined, lumenized constructs. Drawing parallel to liquid droplet interaction, we provide hypothesis on how epithelial organoid assembly can be achieved in a more efficient and predictable manner, of which principles could be extended to other organoid types developed form epithelial stem cells. The guided self-assembly strategy presented here opens up the possibility for biofabricating size-relevant, geometrically defined epithelial structures towards broad applications in biomimetic organoid-devices and tissue engineering.