08:00 | Registration |
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Session Chair: Robin Kettler, University College London |
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Phenotypic Screening |
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09:00 | Advancing Phenotypic Screening Strategies Using Combined Imaging and Pathway Profiling Approaches
Neil Carragher, Principal Investigator, University of Edinburgh, United Kingdom
We demonstrate how high content imaging and Reverse Phase Protein Microarray (RPPA) technologies can combine to enable a robust and unbiased phenotypic drug discovery strategy. We will provide case studies demonstrating how we have applied these technologies to advanced models of cancer to progress new chemical entities and novel drug combinations towards late stage preclinical development.
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09:45 | Analysis of Gene and microRNA Function through High-Content Screening
Miguel Mano, Scientific Manager, International Centre for Genetic Engineering and Biotechnology, Italy
Phenotypic high-content screening enables the systematic testing of large libraries, such as genome-wide libraries of siRNAs and microRNAs, allowing the unbiased analysis of subcellular events on a high number of cells, at the single-cell level. This presentation will discuss examples of the use of high-content screening to characterize gene and microRNA function, in different biological contexts. |
10:30 | Coffee and Networking in Exhibition Hall |
11:00 |  | Keynote Presentation The Value of Phenotypic Screening to Drug Discovery
David Swinney, Chief Executive Officer, Institute for Rare and Neglected Diseases Drug Discovery, United States of America
There is currently a renewed interest to effectively apply phenotypic screening to drug discovery. In this talk I will address why phenotypic screening is of value to successful first-in-class drug discovery and how to realize this value. |
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12:00 |  Technology Spotlight:
Fundacion MEDINA Natural Products Discovery
Francisca Vicente Perez, Area Head, Fundacion Medina
Fundación MEDINA is a non-profit public–private partnership created to discover new compounds and innovative therapies for unmet medical needs. MEDINA offers one of the world’s most productive and diverse natural products libraries from filamentous fungi, actinomycetes, bacteria and plants for discovery of secondary metabolites, making it available screening for pharmaceutical, agricultural, cosmetic and nutritional agents. MEDINA has collective experience with and unbroken historical ties to some of the most important natural products discoveries of recent decades. Therefore, it provides the expertise and tools for maximizing the likelihood of success of screening the collection.
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12:15 | Lunch & Networking in Exhibition Hall |
13:30 | Poster Viewing |
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Session Chair: Kamyar Hadian, HelmholtzZentrum München |
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14:00 |  | Keynote Presentation Selection of Label-free Cellular Imaging Applications for their Integration in the Screening Strategy
Gerardo Turcatti, Director, Ecole Polytechnique Federale De Lausanne, Switzerland
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15:00 | High-Throughput Approaches to Cell Biology
Robin Ketteler, Group Leader/Manager, University College London, United Kingdom
High-throughput screening of autophagy using siRNA and small molecule compounds;
High-content screening of neuronal cells;
Morphometric analysis of secretory granules.
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15:45 | Coffee and Networking in Exhibition Hall |
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RNAi Screening |
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16:15 | A Novel Cell Cycle RNAi Screen using High Content Screening
Stephen Brown, Screening Manager, University of Sheffield, United Kingdom
Most cell cycle screens rely upon an end point assay of a marker, cell number or an enzymatic conversion, each with its own problem. I will discuss a new tool that we have been using for the cell cycle and discuss some of the advantages of this system.
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17:00 | Dissecting Pluripotency with esiRNA Libraries Targeting Protein-coding and Long Non-coding RNAs
Frank Buchholz, Professor, University of Technology Dresden, Germany
Functional screens have greatly extended
our knowledge about pluripotent cells. I will present our efforts to identify protein coding and non-coding transcripts that play a role in pluripotency
maintenance. |
17:45 | Drinks Reception |
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