Jan Markus,
Production Manager/Senior Scientist,
MatTek IVLSL
Jan is responsible for production and further development of human reconstructed tissue models for in vitro testing in MatTek IVLSL. He is also actively involved in the development and validation of new tests aimed at reduction of in vivo testing. His areas of expertise include cell and tissue cultures, molecular biology, virology and oncology. Before joining MatTek IVLSL he worked as senior scientist at Department of Genetics in St. Elizabeth Cancer Institute in Bratislava with primary interests in development and implementation of molecular biology tests for hereditary and sporadic cancers. He has Ph.D. in experimental oncology and his postdoctoral training at National Institutes of Health in Bethesda, USA was aimed at studying the transcriptional regulation in leukemia.
Prediction of Gastrointestinal Toxicity and Drug Absorption Using the Reconstructed 3D Model of Human Small Intestine Epithelium (EpiIntestinal)
Friday, 14 June 2019 at 14:30
Add to Calendar ▼2019-06-14 14:30:002019-06-14 15:30:00Europe/LondonPrediction of Gastrointestinal Toxicity and Drug Absorption Using the Reconstructed 3D Model of Human Small Intestine Epithelium (EpiIntestinal)3D-Culture, Organoids and Tox Screening Europe 2019 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
Small intestine is an important entry gateway for many nutrients, drugs and other substances. About 90% of orally administered drug absorption occurs in the small intestine. Thereby there is a need of good and reliable in vitro model capable to predict drug toxicity and absorption/metabolism patterns that would replace intestine models relying predominantly on the use of cell lines generated from the colon or kidney.
The reconstructed 3D human small intestine model – EpiIntestinal mimics morphology and cell-type composition of normal human small intestine. It is polarized, allows studying bidirectional drug penetration through intestinal wall and expresses proteins involved in active drug transport and metabolism at physiological level. This allows modelling of complex drug absorption profiles, including the permeation, metabolism, drug-drug interaction and adverse effects of drugs on epithelium. Our results revealed that EpiIntestinal mimics the in vivo drug absorption profile much closer than the cancer cell line-based model. EpiIntestinal was also shown to predict toxicity with much higher specificity and sensitivity than the animal model. All in all, this model represents a promising tool to model complex processes occurring in small intestine.
Add to Calendar ▼2019-06-13 00:00:002019-06-14 00:00:00Europe/London3D-Culture, Organoids and Tox Screening Europe 20193D-Culture, Organoids and Tox Screening Europe 2019 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2019-06-14 14:30:002019-06-14 15:30:00Europe/LondonPrediction of Gastrointestinal Toxicity and Drug Absorption Using the Reconstructed 3D Model of Human Small Intestine Epithelium (EpiIntestinal)3D-Culture, Organoids and Tox Screening Europe 2019 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
