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SELECTBIO Conferences 3D-Printing in the Life Sciences

Thomas Angelini's Biography

Thomas Angelini, Professor, Department of Mechanical and Aerospace Engineering, University of Florida

Dr. Thomas E. Angelini is a professor in the Department of Mechanical and Aerospace Engineering at the University of Florida. His research background includes the study of protein, lipid, DNA and virus self-assembly; collective cell migration and force transmission in cell monolayers; bacterial biofilm growth and spreading associated with biosurfactants and extracellular polysaccharide. Currently, his work focuses on cell-assembly and collective motion in 2D and 3D cell populations, 3D bioprinting, and 3D printing soft matter.

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The Bioprinter as a Platform for 3D Cell Biology Experimentation

Tuesday, 15 October 2019 at 16:30

Add to Calendar ▼2019-10-15 16:30:002019-10-15 17:30:00Europe/LondonThe Bioprinter as a Platform for 3D Cell Biology Experimentation3D-Printing in the Life Sciences in Coronado Island, CaliforniaCoronado Island,

The remarkable differences between cells grown on plates and cells in vivo or in 3D culture are well-known. At the physical level, cell shape, structure, motion, and mechanical behavior in 3D are totally different from those in the dish and are far less explored. At the molecular level, cells grown in monolayers exhibit gene expression profiles that do not correlate or are anticorrelated with those of cells grown in 3D culture or xenograft animal models. However, our understanding of cell biology has been heavily shaped by the culture plate, whether viewed through the lens of gene expression profiles, signaling pathways, morphological characterization, or mechanical behaviors. Closing this major gap between 2D in vitro culture and in vivo biology requires a tunable and flexible method for creating 3D cell assemblies and performing experiments on cells in 3D environments. In this talk I will describe how we use a bioprinter in combination with a 3D culture medium made from jammed microgels to perform a wide range of 3D experiments. I will demonstrate this experimental platform’s ability to print structures made from multiple cell types or extracellular matrix with predictable feature sizes down to the scale of a few cell bodies. I will also present data from numerous types of experiments performed in 3D, designed to explore collective cell behavior and cell-cell interactions. For example, I recent results will be presented from a 3D immunotherapy model in which we investigate how immune-specific T cells attack 3D printed brain tumoroids. Our results demonstrate that, in parallel to pursuing the long-standing goals shared by those within the 3D bioprinting field, the current state of bioprinting technology can leveraged to facilitate the emergence and growth of new areas of scientific investigation.

Add to Calendar ▼2019-10-14 00:00:002019-10-15 00:00:00Europe/London3D-Printing in the Life Sciences3D-Printing in the Life Sciences in Coronado Island, CaliforniaCoronado Island,