Profiling CTC Heterogeneity in Patients Using MicrofluidicsTuesday, 17 November 2015 at 10:00 Add to Calendar ▼SELECTBIOenquiries@selectbiosciences.com The analysis of circulating tumor cells (CTCs) is an important capability that may lead to new approaches for cancer management. CTC capture devices developed to date isolate a bulk population of CTCs and do not differentiate subpopulations that may have varying phenotypes with different levels of clinical relevance. Here, we present a new device for CTC spatial sorting and profiling that sequesters blood-borne tumor cells with different phenotypes into discrete spatial bins. Antibody-functionalized magnetic nanoparticles facilitate CTC sorting, and permit deconvolution of phenotypic subpopulations. Working with patient blood samples, we obtain profiles that elucidate the heterogeneity of CTC populations present in cancer patients. Samples from patients undergoing treatment for prostate, breast, and renal cancers have been analyzed using this approach, and samples collected from xenograft cancer models have also been used for validation of the approach. |