Hsueh-Chia Chang,
Bayer Professor of Chemical and Biomolecular Engineering,
University of Notre Dame, Interim Chief Technology Officer, Aopia Biosciences
Over 300 publications, with 18,000 citations and h index of 76. He has confounded a startup Aopia Biosciences in 2020 on purification of extracellular vesicles and currently serves as its interim CTO.
|
|
A Solid-State Nanopore microRNA Quantification PlatformThursday, 16 March 2017 at 11:30 We report a new highly selective (PCR-free) solid-state nanopore miRNA
quantification platform for liquid biopsy and single-cell assays. A
single ion-track nanopore in a PET membrane is asymmetrically etched
into a conic geometry and is coated with a high-permittivity dielectric
layer by Atomic Layer Deposition. The surface modified conic nanopore allows for high
throughput molecular translocation (100 Hz vs 1 Hz for protein
nanopores) and selective delay of single-stranded (ss) nucleic acids
compared to their hybridized double-stranded (ds) duplex (mean
translocation time of 100 ms vs 1 ms with 5% overlap in the two
distributions). The delay is due to enhanced van der Waal attraction
between exposed rings of the ss-nucleic acids, with delocalized
electrons, with the high-permittivity coating. Individual translocation
events can be recorded for a mixture of ss- and ds-nucleic acids
numbering between 100 to 100,000. Whether the translocating molecule of each event is an ss miRNA or its ds duplex can be discerned with 95% confidence.
|
Add to Calendar ▼SELECTBIOenquiries@selectbiosciences.com
 2_150x75.png)
