Enrichment of Fetal DNA Fractions by Microfluidic Extraction of Maternal PlasmaTuesday, 25 March 2014 at 15:30 Add to Calendar ▼2014-03-25 15:30:002014-03-25 16:30:00Europe/LondonEnrichment of Fetal DNA Fractions by Microfluidic Extraction of Maternal PlasmaCirculating Biomarkers 2014 in Boston, Massachusetts, USABoston, Massachusetts, USASELECTBIOenquiries@selectbiosciences.com The presence of circulating fetal nucleic acids in maternal circulation provides a unique opportunity for Non Invasive Prenatal Testing (NIPT) as an alternative to invasive techniques such as amniocentesis or chorionic villus sampling. Prompt and effective enrichment of circulating fetal DNA from blood impact the ability to perform testing. We propose a microfluidic device to rapidly enrich fetal DNA from 2-4mL maternal blood samples. We will show that in plasma extracted on-chip a three-fold average relative amount of fetal DNA (compared with total DNA) was obtained compared to plasma extracted via centrifugation (p=0.02). We therefore demonstrate that a portable microfluidic device can significantly enriches fetal fractions from maternal blood samples in one single step. The operation requires minimal training and does not need large items of specialized equipment. This device may be deployed near-patient to improve the testing accuracy and ability to test NIPT samples with low fetal fractions. |