Kenneth Henry,
Senior Research Scientist,
JSR Micro, Inc.
Dr. Henry began his career receiving a PhD in Genetics at Case Western Reserve University under the guidance of Professor Sandra Lemmon focusing on vesicular trafficking. Following his reception of his doctorate, Dr. Henry worked with Dr. Dean Hamer at the National Institutes of Health developing microbiocides for HIV-1. Advancing his career in HIV-1 research, Dr. Henry worked with a team of researchers from Diagnostic Hybrids Inc. and Dr. Eric Arts laboratory at Case Western Reserve University to develop a HIV-1 Drug Resistance diagnostics. Leaving Case Western Reserve University in 2011, Dr Henry joined the Life Science team at JSR Micro Inc. as a Senior Research Scientist. Utilizing knowledge learned from HIV-1 virions isolation, vesicular trafficking, and RNA isolation techniques, Dr Kenneth Henry is now furthering research and kit development in the realm of exosome and RNA isolation at JSR Micro Inc.
A Rapid and Non-destructive Magnetic Beads-based Exosome Isolation and Enrichment Method
Monday, 24 March 2014 at 14:30
Add to Calendar ▼2014-03-24 14:30:002014-03-24 15:30:00Europe/LondonA Rapid and Non-destructive Magnetic Beads-based Exosome Isolation and Enrichment MethodCirculating Biomarkers 2014 in Boston, Massachusetts, USABoston, Massachusetts, USASELECTBIOenquiries@selectbiosciences.com
In order to isolate exosomes from various body fluids and cell culture supernatants, we have successfully developed ExoCap, which utilizes a magnetic beads based isolation method. ExoCap consists of magnetic particles coupled with antibodies that recognize antigens on the exosome surface, an irrigation solution, and a reagent that releases the captured exosomes for analysis. The antibodies against CD9, CD63, CD81, and EpCAM were specifically selected for this kit. ExoCap can separate easily exosomes within 30 minutes, without ultracentrifuge or any special equipment. A sample amount of 0.1mL is sufficient. In addition, it is an animal free system which is superior to other methods for mass analysis. Moreover, this method enables non-destructive purification of exosomes. To confirm exosomal isolation from diverse body fluids (such as human serum, plasma, urine) and cell culture media, exosomes were examined by western blot, particle size distribution measurement, and scanning transmission electron microscopy (TEM). Exosomes isolated by ExoCap had a lipid bilayer membrane, showed a particle size distribution around 100nm, and expressed tetraspanin molecules.
Authors: Kenneth Henry, Ph.D, Senior Research Scientist, Life Sciences, JSR Micro, Inc. Tetsuji Yamaguchi, Ph.D. JSR Life Sciences
Add to Calendar ▼2014-03-24 00:00:002014-03-25 00:00:00Europe/LondonCirculating Biomarkers 2014Circulating Biomarkers 2014 in Boston, Massachusetts, USABoston, Massachusetts, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2014-03-24 14:30:002014-03-24 15:30:00Europe/LondonA Rapid and Non-destructive Magnetic Beads-based Exosome Isolation and Enrichment MethodCirculating Biomarkers 2014 in Boston, Massachusetts, USABoston, Massachusetts, USASELECTBIOenquiries@selectbiosciences.com
