Aurelio Lorico,
Professor of Pathology,
Touro University Nevada School of Medicine
Aurelio Lorico did his post-doc at Yale University, mentored in the pharmacology field by Alan Sartorelli. Later, as Junior Research Faculty at Yale he clarified the biological function(s) of the MRP1 gene by generating and characterizing MRP1 knockout mice and cell lines. After 8 years at Yale, he became senior scientist at the Norwegian Cancer Center in Oslo, working on cancer drug resistance and designing new strategies for gene therapy of hereditary diseases. Back to the US, he has been working on cell-to-cell communication in the tumor microenvironment, particularly cell-cell fusion and extracellular vesicles, and on the development of innovative therapeutic strategies for breast cancer, melanoma and glioblastoma. His lab has recently discovered the mechanism of nuclear transport of the cargo of extracellular vesicles and has synthesized new drugs that block the transport and have anti-cancer metastatic activity.
Extracellular Vesicles Go Nuclear
Thursday, 28 March 2019 at 17:30
Add to Calendar ▼2019-03-28 17:30:002019-03-28 18:30:00Europe/LondonExtracellular Vesicles Go NuclearExtracellular Vesicle-based Dx and Rx Summit in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Molecular mechanisms regulating EV biogenesis, their release, and
subsequent uptake by target cells have emerged during the last two
decades. How their cargo molecules are selectively delivered to their
intracellular sites of action, including the intra-nuclear compartment,
is still obscure. This issue is particularly important given that the
biogenesis and functionality of EVs are dysregulated under pathological
conditions. Recently, we described a novel sub-nuclear compartment which
is created by the entry of small GTPase Rab7-containing late endosomes
in the nucleoplasmic reticulum. The latter is shaped by superficial and
deep nuclear envelope invaginations (NEI) penetrating into the
nucleoplasm. Given that late endosomes in NEI has often an elongated
appearance and resembles a sword in its scabbard, we proposed to name
this dual-structure “spathasome” from Greek/Latin words “spathi/spatha”
for sword. This structure appears to act as an intermediate compartment
for the delivery of the content of endocytosed EVs (e.g., CD9/CD133
protein complexes and RNA molecules) to the nucleoplasm of their host
cell. The NEI-associated late endosomes and nuclear localization of
EV-derived proteins were observed in cancer cells and mesenchymal
stromal cells in cultures and in breast cancer patient biopsies. A
molecular complex, investigated by indirect immunofluorescence,
fluorescence resonance energy transfer, immunoisolation techniques and
RNA interference, was found to be responsible for the entry of EV cargo
into the nucleoplasmic reticulum.
Add to Calendar ▼2019-03-28 00:00:002019-03-28 00:00:00Europe/LondonExtracellular Vesicle-based Dx and Rx SummitExtracellular Vesicle-based Dx and Rx Summit in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2019-03-28 17:30:002019-03-28 18:30:00Europe/LondonExtracellular Vesicles Go NuclearExtracellular Vesicle-based Dx and Rx Summit in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
