| Conferences \ Circulating Biomarkers World Congress 2016 \ RNA-Seq, Digital PCR, and Liquid Biopsies: Capturing Value from Circulating Biomarkers \ Agenda \ Robert Kitchen |
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Computational Analysis of Exosomal Small-RNA-Seq DataTuesday, 22 March 2016 at 11:00  Add to Calendar ▼2016-03-22 11:00:002016-03-22 12:00:00Europe/LondonComputational Analysis of Exosomal Small-RNA-Seq DataSELECTBIOenquiries@selectbiosciences.comOverview of analysis of small RNAs by RNA-sequencing, including best practices for mitigating nuisance contaminants and special considerations for profiling extra-cellular RNAs (exRNAs). Based on these best practices we present the extracellular RNA processing toolkit (exceRpt), a software suite for the analysis of RNA-seq data obtained from both cellular and extracellular small-RNA preparations. We extend beyond existing analysis methods used to assess cytosolic micro-RNAs (miRNAs) to specifically address experimental issues that may arise in exRNA profiling, such as variable contamination of ribosomal RNAs, the presence of other smallRNA biotypes, and fragmented mRNA and long non-coding RNA transcripts. We also discuss alignments to exogenous miRNAs in miRBase, as well as to exogenous ribosomal RNAs in RDP for comparison with studies of the microbiome. |
Add to Calendar ▼2016-03-21 00:00:002016-03-22 00:00:00Europe/LondonRNA-Seq, Digital PCR, and Liquid Biopsies: Capturing Value from Circulating BiomarkersSELECTBIOenquiries@selectbiosciences.com
