Charalampos Tzivelekis's Biography

Charalampos Tzivelekis, Researcher, Newcastle University

Babis Tzivelekis is a researcher in the School of Engineering at Newcastle University (UK). He is researching the rapid manufacturing of microfluidic devices and instrumentation for DNA diagnosis with innovative, low-cost stereolithography 3D printing and high-precision machining techniques. During his PhD he has worked extensively on the design, manufacture, characterisation and optimisation of polymer lab-on-chip platforms to process DNA based assays for Polymerase Chain Reaction (PCR). His research is co-funded by the Engineering and Physical Sciences Research Council (EPSRC) Centre for Doctoral Training in Additive Manufacturing and QuantuMDx, Ltd.

Fabrication via DLP® Stereolithography and Characterization of Microfluidic Cartridges Suitable for Polymerase Chain Reaction (PCR)

Wednesday, 19 June 2019 at 18:15

Add to Calendar ▼2019-06-19 18:15:002019-06-19 19:15:00Europe/LondonFabrication via DLP® Stereolithography and Characterization of Microfluidic Cartridges Suitable for Polymerase Chain Reaction (PCR)Lab-on-a-Chip and Microfluidics Europe 2019 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com

DLP® Stereolithography (SLA) as a high-resolution 3D printing process offers a low-cost alternative for prototyping of polymer microfluidic devices, compared to common fabrication methods. Here, we introduce specific processing routes of DLP® SLA in fabrication of microfluidic devices suitable for Polymerase Chain Reaction (PCR). A fabrication protocol for cartridges with monolithic 2.5D micro-channels was evaluated in the production of disposable flow-through and stationary PCR devices. Those were interfaced with purpose-built PCR thermocyclers and evaluated in-situ by amplification of a 75 bp target sequence from a genomic DNA template. PCR inhibition sources were further analyzed with qPCR. Considering the low unit cost per device and the capability to endow further functionality on printed devices through surface modification, we further examined hydrophobic tuning protocols and analysed their performance in-situ. We finally summarize the advantages of DLP SLA and characterize its potential as a future fabrication technique of lab-on-chip devices.

Add to Calendar ▼2019-06-18 00:00:002019-06-19 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics Europe 2019Lab-on-a-Chip and Microfluidics Europe 2019 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com

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