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Next Generation Multiplexed Subset Serology Screening for Auto-Immune Disorders using Grace Bio-Labs™ ArrayCAM™ Platform

Thursday, 18 September 2014 at 17:15

Add to Calendar ▼2014-09-18 17:15:002014-09-18 18:15:00Europe/LondonNext Generation Multiplexed Subset Serology Screening for Auto-Immune Disorders using Grace Bio-Labs™ ArrayCAM™ PlatformLab-on-a-Chip, Microfluidics and Microarray World Congress in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com

Greater than 20 million Americans are afflicted by over 80 clinically distinct auto-immune diseases, an immune system dysfunction in which the body attacks its own organs, tissues and cells.  Systemic autoimmune diseases such as Systemic lupus erythematosus, Sjorgren syndrome and others are a specific subset of diseases tested via immunofluorescence anti-nuclear antibody (ANA) screens followed by a cascade of reflex tests to identify multiple sub-set markers.  Testing with numerous standard IF or EIA assays exhibit many issues including interpretation differences in techniques as well as the sensitivity and specificity across the myriad of different vendor platforms and methods causing variability.  The microarray platform is particularly well suited to profiling multiple markers because the numerous analytes are measured on a fixed platform within a single sample thereby reducing ambiguous measurements or erroneous interpretations.  Here we describe a robust, low cost, high throughput method for screening multiple ANA reflex markers within a single sample well.  Our method consists of recombinant antigen arrays printed on Grace Bio-Labs ONCYTE® porous nitrocellulose film slides and employs multiple quantum nano-particle (QNP) detection probes imaged with the ArrayCAM™ instrument.  This platform provides high signal to noise measurements driven by the native protein orientation and high binding capacity of the ONCYTE PNC film substrate coupled with the spectrally distinct wavelengths of the QNP detector dyes and ArrayCAM reader.  The advantages of this system over IF or EIA for a clinical laboratory include significant laboratory time savings, internal sample normalization, low cost instrumentation, familiar and easy to use protocols while providing sensitivity/specificity capabilities commensurate with clinical lab standards.