Charles Henry,
Professor and Chair,
Colorado State University
Charles Henry is a Professor in and Chair of the Chemistry Department at Colorado State University. He also holds appointments in Chemical & Biological Engineering and Biomedical Engineering. Work in the Henry group focuses on the application of microfluidics to environmental and occupational exposure monitoring, measurement of chemical gradients in biology, and development of low cost, point of need measurement systems.
Biological Imaging Using Microfluidics and Electrochemistry
Wednesday, 28 September 2016 at 09:30
Add to Calendar ▼2016-09-28 09:30:002016-09-28 10:30:00Europe/LondonBiological Imaging Using Microfluidics and ElectrochemistrySELECTBIOenquiries@selectbiosciences.com
Chemical gradients drive many processes in biology, ranging from nerve
signal transduction to ovulation to cancer metastasis. At present,
microscopy is the primary tool used to understand these gradients by
imaging both the gradients and the resulting cell motion. Microscopy has
provided many important breakthroughs in our understanding of
fundamental biology, but is limited due to the need to incorporate
fluorescent molecules into biological systems through either labeling or
genetic manipulations. To better understand some processes, there is a
need to develop tools that can measure chemical gradient formation in
biological systems that do not require fluorescent modification of the
targets, can be multiplexed to measure more than one molecule, and are
compatible with a variety of biological sample types, including in vitro
cell cultures and ex vivo tissue slices. In response to this need, we
have developed a high-density electrode array containing 8,192
individual electrodes to image release of electrochemically active
metabolites like nitric oxide and norepinephrine from live tissue
slices. The electrode array has a resolution of 30 µm and covers and
area of 2 mm by 2 mm, easily large enough to image release of
neurotransmitters across an ex vivo tissue slice from a mouse model. In
this talk, electrochemical characterization of this system will be
discussed first using simple chemical models. Then use of the system in
combination with microfluidics, for imaging spatiotemporal resolution of
neurotransmitter release at the organ scale will be presented. Use of
microfluidics to deliver stimulants that induce changes in release
profile will be shown.
Add to Calendar ▼2016-09-26 00:00:002016-09-28 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics: Emerging Themes, Technologies and ApplicationsSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2016-09-28 09:30:002016-09-28 10:30:00Europe/LondonBiological Imaging Using Microfluidics and ElectrochemistrySELECTBIOenquiries@selectbiosciences.com
