Shoji Takeuchi,
Professor, Center For International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science,
The University of Tokyo
Shoji Takeuchi received the B.E, M.E., and Dr. Eng. degrees in mechanical engineering from the University of Tokyo, Tokyo, Japan, in 1995, 1997, and 2000, respectively. He is currently a Professor in the Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), University of Tokyo. Since 2008, he is a director of Collaborative Research Center for Bio/Nano Hybrid Process at IIS. His current research interests include membrane protein chips, bottom-up tissue engineering and biohybrid MEMS. He received several awards including Young Scientists' Prize, the Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology in 2008, the JSPS prize from the Japan Society for the Promotion of Science in 2010.
In this presentation, I will talk about several Microfluidic-based approaches for the rapid construction of 3D-cellular construct. Large-scale 3D tissue architectures that mimic microscopic tissue structures in vivo are very important for not only in tissue engineering but also drug development without animal experiments. We demonstrated a construction method of 3D tissue structures by using cell beads and cell fibers. To prepare the cellular beads, we used an axisymmetric flow focusing device (AFFD) that allows us to encapsulate cells within monodisperse collagen beads. By molding these cell beads into a 3D chamber and incubating them, we successfully obtained complicated and milli-sized 3D cellular constructs. As the cell fibers, a cell-encapsulating core-shell hydrogel fiber was produced in a double coaxial laminar flow microfluidic device. When with myocytes, endothelial, and nerve cells, they showed the contractile motion of the myocyte cell fiber, the tube formation of the endothelial cell fibers and the synaptic connections of the nerve cell fiber, respectively. By reeling, weaving and folding the fibers using microfluidic handling, higher-order assembly of fiber-shaped 3D cellular constructs can be performed. Moreover, the fiber encapsulating beta-cells is used for the implantation of diabetic mice, and succeeded in normalizing the blood glucose level.
In this presentation, I will talk about several Microfluidic-based
approaches for the rapid construction of 3D-cellular construct.
Large-scale 3D tissue architectures that mimic microscopic tissue
structures in vivo are very important for not only in tissue engineering
but also drug development without animal experiments. We demonstrated a
construction method of 3D tissue structures by using cell beads and
cell fibers. To prepare the cellular beads, we used an axisymmetric flow
focusing device (AFFD) that allows us to encapsulate cells within
monodisperse collagen beads. By molding these cell beads into a 3D
chamber and incubating them, we successfully obtained complicated and
milli-sized 3D cellular constructs. As the cell fibers, a
cell-encapsulating core-shell hydrogel fiber was produced in a double
coaxial laminar flow microfluidic device. When with myocytes,
endothelial, and nerve cells, they showed the contractile motion of the
myocyte cell fiber, the tube formation of the endothelial cell fibers
and the synaptic connections of the nerve cell fiber, respectively. By
reeling, weaving and folding the fibers using microfluidic handling,
higher-order assembly of fiber-shaped 3D cellular constructs can be
performed. Moreover, the fiber encapsulating beta-cells is used for the
implantation of diabetic mice, and succeeded in normalizing the blood
glucose level.
Add to Calendar ▼2017-10-02 00:00:002017-10-04 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics World Congress 2017Lab-on-a-Chip and Microfluidics World Congress 2017 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼SELECTBIOenquiries@selectbiosciences.com
