| Conferences \ Lab-on-a-Chip & Microfluidics World Congress 2017 \ Lab-on-a-Chip and Microfluidics: Companies, Technologies and Commercialization \ Agenda \ Hsueh-Chia Chang |
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PCR-Free MicroRNA Quantification Based on Ion-Selective Nanoporous Membranes and NanoporesMonday, 2 October 2017 at 17:15  Add to Calendar ▼2017-10-02 17:15:002017-10-02 18:15:00Europe/LondonPCR-Free MicroRNA Quantification Based on Ion-Selective Nanoporous Membranes and NanoporesSELECTBIOenquiries@selectbiosciences.comWe report an integrated biochip platform that can identify and quantify low copy numbers of microRNA biomarkers in a heterogeneous physiological sample like blood, saliva or urine. This quantification assay is done without PCR amplification, reporter labeling, extensive off-chip pretreatment and expensive optical sensors. Consequently, it does not introduce PCR/ligation bias and limits analyte loss during pretreatment. The main components of the integrated biochips are nanoporous membranes and solid-state nanopores with pore radii smaller than the nm-scale Debye length. We use the ion concentration and charge polarization features of the ion-selective membranes to control the on-chip ionic strength, actuate pH by splitting water, lyse exosomes and isolate/concentrate the target molecules. The final nanopore sensor or sensor array utilizes surface modification and pore geometry to preferentially delay the translocation time of the target microRNAs to achieve single-molecule identification and quantification. The integrated chip achieves a translocation frequency (throughput) that is at least one hundred times higher than any literature or commercial nanopore technology. |
Add to Calendar ▼2017-10-02 00:00:002017-10-04 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics: Companies, Technologies and CommercializationSELECTBIOenquiries@selectbiosciences.com
