A Systematic Comparison of Single Cell RNA-Seq MethodsThursday, 18 March 2021 at 13:30 Add to Calendar ▼2021-03-18 13:30:002021-03-18 14:30:00Europe/LondonA Systematic Comparison of Single Cell RNA-Seq MethodsInnovations in Microfluidics and SCA 2021 in BostonBostonSELECTBIOenquiries@selectbiosciences.com A multitude of single-cell RNA sequencing methods have been developed in recent years, with dramatic advances in scale and power, and enabling major discoveries and large scale cell mapping efforts. We directly compared seven methods for single cell and/or single nucleus profiling from three types of samples – cell lines, peripheral blood mononuclear cells and brain tissue. To analyze these datasets, we developed and applied scumi, a flexible computational pipeline that can be used for any scRNA-seq method. We evaluated the methods for both basic performance and for their ability to recover known biological information in the samples. |