Lydia Sohn,
Almy C. Maynard and Agnes Offield Maynard Chair in Mechanical Engineering,
University of California-Berkeley
Lydia L. Sohn received her A.B. (Chemistry and Physics, 1988), M.S. (Physics, 1990), and Ph.D. (Physics, 1992) from Harvard University. She was an NSF/NATO postdoc at Delft University of Technology and a postdoc at AT&T Bell Laboratories (1993-1995). Sohn was an Assistant Professor of Physics at Princeton University prior to joining the Mechanical Engineering Dept. at UC Berkeley in 20013. Her work focuses on developing quantitative techniques to probe single cells. Sohn has received numerous awards including the NSF CAREER, Army of Research Young Investigator Award, DuPont Young Professor Award, and a Bakar Fellowship. In 2014, she was one of five winners in the “Identifying Platform Technologies for Advancing Life Sciences Research” competition for her work on Node-Pore Sensing. Most recently, she was elected a Fellow of the American Institute for Medical and Biological Engineering.
mechano-NPS: An Electronic Method to Mechanically Phenotype Cells
Monday, 21 March 2022 at 10:00
Add to Calendar ▼2022-03-21 10:00:002022-03-21 11:00:00Europe/Londonmechano-NPS: An Electronic Method to Mechanically Phenotype CellsInnovations in Microfluidics and SCA 2022 in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
We have developed an efficient, label-free method of screening cells for their phenotypic profile, which we call Node-Pore Sensing (NPS). NPS involves measuring the modulated current pulse caused by a cell transiting a microfluidic channel that has been segmented by a series of inserted nodes. Previously, we showed that when segments between the nodes are functionalized with different antibodies corresponding to distinct cell-surface antigens, immunophenotyping can be achieved. In this talk, I will show how we have significantly advanced NPS by simply inserting between two nodes a “contraction” channel through which cells can squeeze. “Mechano-NPS”, as we now call our method, can simultaneously measure a cell’s size, stiffness, and ability to recover from deformation. We have used mechano-NPS to assess the mechanical properties of acute promyelocytic leukemia (APL) cells. APL is an acute myeloid leukemia subtype for which all-trans retinoic acid (ATRA) is an essential therapy. 20% of APL patients are resistant to ATRA, which must be administered during the acute phase of the disease to prevent death. We show that ATRA resistant APL cells are less mechanically pliable than ATRA-responsive cells. Thus, a potential biomarker for APL resistance may ultimately be mechanical stiffness.
Add to Calendar ▼2022-03-21 00:00:002022-03-22 00:00:00Europe/LondonInnovations in Microfluidics and SCA 2022Innovations in Microfluidics and SCA 2022 in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2022-03-21 10:00:002022-03-21 11:00:00Europe/Londonmechano-NPS: An Electronic Method to Mechanically Phenotype CellsInnovations in Microfluidics and SCA 2022 in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
