Haim H. Bau is a professor of Mechanical Engineering and Applied Mechanics at the University of Pennsylvania.
Carbon Nanopipettes (CNPs) for Automated Microinjection and the Study of Sub-cellular tRNA Dynamics
Tuesday, 29 September 2015 at 13:30
Add to Calendar ▼2015-09-29 13:30:002015-09-29 14:30:00Europe/LondonCarbon Nanopipettes (CNPs) for Automated Microinjection and the Study of Sub-cellular tRNA DynamicsNGS, SCA, Mass Spec: The Road to Diagnostics in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
We describe the use of carbon nanopipettes (CNPs) for microinjection into individual cells. The CNP consists of a pulled-quartz micropipette with a thin layer of carbon deposited along its entire interior surface. The tip of the CNP is etched to expose a carbon pipe that varies in diameter from tens to hundreds of nm. The bore of the CNP facilitates microinjection, while the carbon film enables electrical measurements. We utilize the carbon film for impedimetric detection of cytoplasmic and nuclear penetration to trigger injection. We use the CNPs in a custom-developed, automated injection system. As a demonstration of an application, we describe microinjection of fluorescently-labeled tRNA to monitor subcellular tRNA dynamics in real time.
Add to Calendar ▼2015-09-28 00:00:002015-09-30 00:00:00Europe/LondonNGS, SCA, Mass Spec: The Road to DiagnosticsNGS, SCA, Mass Spec: The Road to Diagnostics in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2015-09-29 13:30:002015-09-29 14:30:00Europe/LondonCarbon Nanopipettes (CNPs) for Automated Microinjection and the Study of Sub-cellular tRNA DynamicsNGS, SCA, Mass Spec: The Road to Diagnostics in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
