Single Cell Random Access MemoryTuesday, 29 September 2015 at 17:00 Add to Calendar ▼2015-09-29 17:00:002015-09-29 18:00:00Europe/LondonSingle Cell Random Access MemoryNGS, SCA, Mass Spec: The Road to Diagnostics in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com The ability to manipulate small fluid droplets, colloidal particles and single cells with the precision and parallelization of modern-day computer hardware has profound applications for biochemical detection, gene sequencing, chemical synthesis and highly parallel analysis of single cells. Drawing inspiration from general circuit theory and magnetic bubble technology, we have recently demonstrated a class of integrated circuits for executing sequential and parallel, timed operations on an ensemble of single particles and cells. The integrated circuits are constructed from lithographically defined, overlaid patterns of magnetic film and current lines. The magnetic patterns passively control particles similar to electrical conductors, diodes and capacitors. The current lines actively switch particles between different tracks similar to gated electrical transistors. When combined into arrays and driven by a rotating magnetic field clock, these integrated circuits have general multiplexing properties and enable the precise control of magnetizable objects. The presentation will focus on our recent progress on optimizing these integrated circuits to manipulate magnetically labeled CD4+ T cells, including recent data on single cell switches, cell velocities on chip, as well as the ability of non-fouling surface coatings to resist non-specific adhesion between the cells and chip surface. |