Dr. Sirenko is an established Cell Biologist and Imaging Specialist who is an international expert in development of assays with complex cell-based models for research and drug discovery. She is a Senior Scientist at Molecular Devices where she has worked extensively with High Content Imaging and its application to analysis of novel cell systems. Dr. Sirenko currently leads a group of scientists and engineers developing methods and new tools for automation of 3D cell models – including organoids and organ-on chips – for modeling cancer, neurotoxicity, cardiac diseases and toxicology. She is also actively involved in establishing collaborations in the areas of cell culture, microfluidics, and image analysis. She has over 15 years of industry experience, holds a PhD in Biochemistry/Biophysics, and has authored more than 36 scientific papers in the fields of cell biology, toxicology, and biotechnology.
Organoids For Disease Modeling and In Vitro Assessment of Drug Effects by High-Content Imaging and 3D Analysis
Monday, 22 March 2021 at 14:00
Add to Calendar ▼2021-03-22 14:00:002021-03-22 15:00:00Europe/LondonOrganoids For Disease Modeling and In Vitro Assessment of Drug Effects by High-Content Imaging and 3D Analysis3D-Culture, Organoids and Organs-on-Chips 2021 in SELECTBIOenquiries@selectbiosciences.com
3D cell models representing different tissues were successfully used for studying complex biological effects, tissue architecture, and functionality. While complexity of 3D models remains a hurdle for the wider adoption in research and drug screening, significant progress has been made in developing tools to automate the culture, monitoring, and analysis of these structures. This enabled an increase in quality of information, throughput, and precision of complex biological assays. We describe automated cell culture and high-content imaging methods that allow monitoring and characterization of growth and differentiation of lung organoids, as well as toxicity and drug effects. Increase in size and complexity of developing organoids was monitored during eight weeks of development. We demonstrated concentration-dependent toxicity effects of several drugs that have been known to cause lung damage. In addition, responses to several anti-cancer drugs were monitored in 3D tumoroids derived from primary tumor tissues. Advanced image analysis allowed 3D reconstitution and complex analysis of organoid structures, characterization of cell morphology and viability, as well as determining expression level of differentiation markers. Image analysis included traditional and Machine Learning-based tools to increase the ability to discern important biological information from the complex multi-dimensional datasets. These quantitative characterization of organoids morphology can be used for studying disease phenotypes and compound effects in complex assay models.
Add to Calendar ▼2021-03-22 00:00:002021-03-23 00:00:00Europe/London3D-Culture, Organoids and Organs-on-Chips 20213D-Culture, Organoids and Organs-on-Chips 2021 in SELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2021-03-22 14:00:002021-03-22 15:00:00Europe/LondonOrganoids For Disease Modeling and In Vitro Assessment of Drug Effects by High-Content Imaging and 3D Analysis3D-Culture, Organoids and Organs-on-Chips 2021 in SELECTBIOenquiries@selectbiosciences.com
