Mark Rosowski,
Assistant Professor and Head of Tissue Engineering & Molecular Biology,
Technische Universität Berlin
Diploma in Biochemistry, FU Berlin. Doctoral Degree (rer. nat.), TU Berlin – focus: skeletal tissue engineering. Since 2008 Assistant Professor and Head of Tissue Engineering & Molecular Biology Technische Universität Berlin Institute of Biotechnology; Medical Biotechnology.
Bone Marrow-on-a-Chip: Long-Term Culture of Human Hematopoietic Stem Cells in a 3D-Microfluidic Environment
Wednesday, 6 June 2018 at 16:30
Add to Calendar ▼2018-06-06 16:30:002018-06-06 17:30:00Europe/LondonBone Marrow-on-a-Chip: Long-Term Culture of Human Hematopoietic Stem Cells in a 3D-Microfluidic EnvironmentOrgan-on-a-Chip, Tissue-on-a-Chip Europe 2018 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
Multipotent hematopoietic stem cells (HSCs) give rise to all blood cell types. The bone marrow stem cell niche in which the HSCs are maintained is known to be vital for their sustainment. Unfortunately, to this date, no in vitro model exists that truthfully mimics the aspects of the bone marrow niche and simultaneously allows the long-term culture of HSCs. In this study, we present a novel 3D co-culture model, based on a hydroxyapatite-coated zirconium oxide scaffold mimicking the trabecular bone. Primary human mesenchymal stromal cells (MSCs) were employed to build up a suitable environment within the scaffold for the culture of HSCs. Subsequently, primary human HSCs were seeded in this bone marrow mimicking environment. The whole culture was carried out within a microfluidic multi-organ-chip platform (2OC). Immunofluorescent staining, qPCR, and scanning electron microscopy data demonstrated that the MSCs had produced an appropriate environment for HSC cultivation. Using flow cytometry and CFU-GEMM assays, it could be shown that HSCs remained their native phenotype for at least eight weeks. Moreover, cells cultured for four weeks in the bone marrow model passed the gold standard test for native multipotent HSCs by successfully engrafting in irradiated immunocompromised mice. Here we present for the first time a novel human in vitro bone marrow model, capable of the successfully long-term culture of functional multipotent HSCs in a microfluidic environment.
Add to Calendar ▼2018-06-05 00:00:002018-06-06 00:00:00Europe/LondonOrgan-on-a-Chip, Tissue-on-a-Chip Europe 2018Organ-on-a-Chip, Tissue-on-a-Chip Europe 2018 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2018-06-06 16:30:002018-06-06 17:30:00Europe/LondonBone Marrow-on-a-Chip: Long-Term Culture of Human Hematopoietic Stem Cells in a 3D-Microfluidic EnvironmentOrgan-on-a-Chip, Tissue-on-a-Chip Europe 2018 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
