Wenjin Xiao,
Postdoctoral Fellow,
Centre de Recherche des Cordeliers, Université Paris Cité
Dr Wenjin Xiao obtained her PhD in chemical system engineering from The University of Tokyo in 2014. In 2018, she was granted a postdoctoral fellowship from Cancer Research and Personalized Medicine (CARPEM) and joined Dr Valerie Taly’s group at Université Paris Cité. She is co-author of 13 publications, including 8 first and/or corresponding authored papers, and has 2 international patents. She has a strong background in cell biology and biochemistry, and expertise in tissue engineering and in vitro models. She has recently acquired experience in the field of cancer biology and tumour heterogeneity.
3D Multicellular Tumor Spheroids to Study Intra-Tumor Heterogeneity and Cell Communication In Colorectal Cancer
Wednesday, 22 June 2022 at 12:30
Add to Calendar ▼2022-06-22 12:30:002022-06-22 13:30:00Europe/London3D Multicellular Tumor Spheroids to Study Intra-Tumor Heterogeneity and Cell Communication In Colorectal CancerOrganoids and Organs-on-Chips Europe 2022 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
The major underlying cause for colorectal cancer (CRC) high mortality rate relies on its drug resistance, to which intra-tumor heterogeneity (ITH) contributes extensively. However, the impact of ITH on CRC evolution and drug resistance remains underexplored. Multicellular tumour spheroids (MCTSs) have been highlighted as pertinent in vitro cancer models, since they are able to more accurately mimic both the 3D structure of malignant tissue and the cancer microenvironment compared to the traditional 2D format. Here, we describe a 3D spheroid model that could mimick the ITH of CRC to study the effect of heterogeneous inter-cellular communication. These MCTSs were composed of CRC cell lines with distinct molecular subtypes, which were capable to grow into a physiological relevance size in a 4-day culture. We observed the specific spatial distribution of each cell population within the spheroids, in line with the observations in tumours. Interestingly, co-cultures did not alter the cell growth in the MCTSs, but significantly sustained the cellular survival in response to a front-line chemotherapeutic drug, 5-Fluorouracil (5-FU). This phenomenon can be supported by the cell secretome, which showed not only remarkable protective effect against 5-FU treatments, but also sustained cell migration and invasion capacity, suggesting that direct cell-cell interaction is not key in this cell communication. Overall, our MCTS model shows a possibility to mimic ITH and to study the therapy-induced cell interplay in vitro, which could potentially stimulate patient CRC progression and in this manner reduce chemotherapy efficacy.
Add to Calendar ▼2022-06-21 00:00:002022-06-22 00:00:00Europe/LondonOrganoids and Organs-on-Chips Europe 2022Organoids and Organs-on-Chips Europe 2022 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2022-06-22 12:30:002022-06-22 13:30:00Europe/London3D Multicellular Tumor Spheroids to Study Intra-Tumor Heterogeneity and Cell Communication In Colorectal CancerOrganoids and Organs-on-Chips Europe 2022 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
