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Ultra-sensitive VFA-based Rapid Detection of Interleukin 6 for Inflammatory Disease

Tuesday, 14 December 2021 at 13:15

Add to Calendar ▼2021-12-14 13:15:002021-12-14 14:15:00Europe/LondonUltra-sensitive VFA-based Rapid Detection of Interleukin 6 for Inflammatory DiseasePoint-of-Care Diagnostics and Biosensors 2021 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com

Increased IL-6 has been reported in chronic diseases such as Rheumatoid Arthritis, cytokine release syndrome (CRS) and sepsis. This work seeks to provide healthcare with a real-time vertical flow assay monitor for tracking IL-6 levels, for early diagnosis and potential IL-6 blockade therapy. After a thorough screening of sizes of gold-NP, membranes, and buffers, the detection of recombinant IL-6 in spiked buffer was found to have a limit of detection of 10 pg/ml and a reportable range of 10-10,000 pg/ml within 15 min assay time. The detection of IL-6 in spiked pooled healthy serum has a LoD of 3.2 pg/ml and a reportable range of 10-10,000 pg/ml. VFA cartridge stability was assessed by comparing one-day, two-week, four-week and six-week storage at room temperature. The standard curves were built under each condition to demonstrate the impact of storage on cartridge stability. Inter-operator CV was assessed by building three standard curves among three researchers and the intra-operator CV was assessed by building three standard curves by one researcher, demonstrating 14.3% and 15.2% CV, respectively. This ultra-sensitive assay can be used for monitoring IL-6 levels for treatment adjustments and assessment of the need for ICU admission or to prognosticate.