Restriction Cascade Exponential Amplification (RCEA): A Novel Bio-detection Technology with Sensitivity Similar to PCR and Near-Absolute SpecificityFriday, 19 September 2014 at 13:30  Add to Calendar ▼2014-09-19 13:30:002014-09-19 14:30:00Europe/LondonRestriction Cascade Exponential Amplification (RCEA): A Novel Bio-detection Technology with Sensitivity Similar to PCR and Near-Absolute SpecificityPoint-of-Care Diagnostics World Congress in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.comThis novel technology achieves selective and sensitive detection of nucleic acid targets by using signal amplification, rather than by amplifying DNA targets as in standard PCR. The RCEA assay is based on the specific cleavage per-formed by restriction endonuclease enzymes and provides for near absolute specificity since two bio-recognition events must occur independently to generate a signal first by hybridization of target DNA to oligonucleotide probes and then restrictase recognition and cleavage of a se-quence-specific locus within the formed double-stranded hybrid. The assay has been demonstrated with the detection of the methicillin-resistant Stapphylococus aureus (MRSA) pathogen, using the mecA gene as the target. The RCEA based assay can be integrated into a variety of user friendly POC formats ranging from instrument-free colorimetric kits to hand-held electrochemical detectors. The assay can be easily adapted to detect any DNA target of interest for POC diagnostics applications. |
Add to Calendar ▼2014-09-18 00:00:002014-09-19 00:00:00Europe/LondonPoint-of-Care Diagnostics World CongressPoint-of-Care Diagnostics World Congress in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
