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gBlocks™ Gene Fragments—Sequence-Verified PCR Fragments

Wednesday, 5 September 2012 at 15:15

Add to Calendar ▼2012-09-05 15:15:002012-09-05 16:15:00Europe/LondongBlocks™ Gene Fragments—Sequence-Verified PCR FragmentsSELECTBIOenquiries@selectbiosciences.com

Integrated DNA Technologies has recently introduced a new molecular biology tool, gBlocks™ Gene Fragments. gBlocks fragments are double-stranded, sequence-verified DNA up to 500 base pairs in length. gBlocks Gene Fragments can be used as copy number standards for qPCR, without the need for cloning the endogenous amplicon. Because the average qPCR amplicon is less than 150 bp, multiple amplicons can be incorporated into a single gBlocks fragment, which is especially useful for multiplex assay controls. A single gBlocks fragment containing multiple target sequences avoids the need for linearizing plasmid controls, and ensures that concentrations of the standard dilutions are the same for the individual control assays in the multiplex reaction.