Protein and RNA Interactors of Human Argonaute ProteinsTuesday, 4 September 2012 at 14:45 Add to Calendar ▼2012-09-04 14:45:002012-09-04 15:45:00Europe/LondonProtein and RNA Interactors of Human Argonaute ProteinsSELECTBIOenquiries@selectbiosciences.com microRNAs (miRNAs) are small non-coding RNAs that function in literally all cellular processes. miRNAs interact with Argonaute (Ago) proteins and guide them to specific target sites located in the 3’ untranslated region (UTR) of target mRNAs leading to translational repression and deadenylation-induced mRNA degradation. Most miRNAs are processed from hairpin-structured precursors by the consecutive action of the RNase III enzymes Drosha and Dicer. However, processing of miR-451 is Dicer-independent and cleavage is mediated by the endonuclease Ago2. Here we have characterized miR-451 sequence and structure requirements for processing as well as sorting of miRNAs into different Ago proteins. |