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Extracellular Vesicles (EVs)-derived exRNA as Liquid biopsy Using NGS

Monday, 9 September 2019 at 17:30

Add to Calendar ▼2019-09-09 17:30:002019-09-09 18:30:00Europe/LondonExtracellular Vesicles (EVs)-derived exRNA as Liquid biopsy Using NGSCirculating Biomarkers, Exosomes and Liquid Biopsy Asia 2019 in Seoul, KoreaSeoul, KoreaSELECTBIOenquiries@selectbiosciences.com

MicroRNAs are small non-coding genes that regulate numerous pathways through targeting the number of mRNAs. Dysregulation of miRNAs including genomic alteration, DNA methylation, transcriptional regulation and abnormal miRNA biogenesis are reported in most cancers. miRNA also found in body fluids including blood, salvia and urea, as important sources for early detection of cancer. Therefore, circulating microRNA may serve as novel diagnostic tools to detect early cancer as well as other diseases. We examined the expression of microRNA using serum/plasma samples obtained from pancreatic cancer patients, head and neck cancer patients hepatic carcinoma cell patients compared with healthy controls. To identify significant biomarker from body fluids, proper normalization strategy is of critical importance. We used microRNA array, qRT-PCR and next generation sequencing (NGS) platform to analyze microRNA, and found that appropriate normalized microRNAs in each platform. We identified a number of biomarker candidates such as mature miRNAs, isomiR, tRNA-derived fragments (tRFs), and other ncRNA, are known to regulate expression of genes involved in cell metabolism and are released into body fluid from various cells with extracellular vesicles. To test the possibilities that these candidate small RNAs are secreted from cancer cells, we purified EVs from serum in health volunteer and cancer patients, and analyzed small RNAs using NGS, and found that some of candidate small RNAs can be found in EVs. Interestingly, these small RNAs are also found in culture media in breast cancer cell lines. Based on our alogism, we identified significant biomarker secreted from cancer cells for early detection of cancer.