Lydia Sohn,
Almy C. Maynard and Agnes Offield Maynard Chair in Mechanical Engineering,
University of California-Berkeley
Lydia L. Sohn received her A.B. (Chemistry and Physics, 1988), M.S. (Physics, 1990), and Ph.D. (Physics, 1992) from Harvard University. She was an NSF/NATO postdoc at Delft University of Technology and a postdoc at AT&T Bell Laboratories (1993-1995). Sohn was an Assistant Professor of Physics at Princeton University prior to joining the Mechanical Engineering Dept. at UC Berkeley in 20013. Her work focuses on developing quantitative techniques to probe single cells. Sohn has received numerous awards including the NSF CAREER, Army of Research Young Investigator Award, DuPont Young Professor Award, and a Bakar Fellowship. In 2014, she was one of five winners in the “Identifying Platform Technologies for Advancing Life Sciences Research” competition for her work on Node-Pore Sensing. Most recently, she was elected a Fellow of the American Institute for Medical and Biological Engineering.
Linking Physical Phenotype to Drug Resistance: Single-Cell Mechanical Measurements of Acute Promyelocytic Leukemia
Wednesday, 9 October 2019 at 10:30
Add to Calendar ▼2019-10-09 10:30:002019-10-09 11:30:00Europe/LondonLinking Physical Phenotype to Drug Resistance: Single-Cell Mechanical Measurements of Acute Promyelocytic LeukemiaSingle Cell Analysis Summit 2019 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
We have developed an efficient, label-free method of screening cells for their phenotypic profile, which we call Node-Pore Sensing (NPS). NPS involves measuring the modulated current pulse caused by a cell transiting a microfluidic channel that has been segmented by a series of inserted nodes. By simply inserting between two nodes a “contraction” channel through which cells can squeeze, we can use NPS to measure simultaneously physical properties of cells, including size, resistance to deformation, transverse deformation, and ability to recover from deformation. “Mechano-NPS”, as we now call our method, can distinguish malignant from non-malignant epithelial cells and discriminate between sub-lineages and, chronological age groups of primary human mammary epithelial cells. As I will discuss, we have recently been using mechano-NPS to study acute promyelocytic leukemia (APL) cells, showing that mechanical phenotyping is predictive of the leukemia’s response to retinoic acid (RA), a first-line chemotherapeutic for APL. By measuring cell recovery, mechano-NPS provides information on a cell’s viscoelastic behavior. While this confers the advantage of making more in-depth biophysical analyses of single cells, it also potentially provides new information to be included in a diagnostic classification.
Add to Calendar ▼2019-10-09 00:00:002019-10-09 00:00:00Europe/LondonSingle Cell Analysis Summit 2019Single Cell Analysis Summit 2019 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2019-10-09 10:30:002019-10-09 11:30:00Europe/LondonLinking Physical Phenotype to Drug Resistance: Single-Cell Mechanical Measurements of Acute Promyelocytic LeukemiaSingle Cell Analysis Summit 2019 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
