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Nanoparticle Flow Cytometry of Individual Extracellular Vesicles

Friday, 19 September 2014 at 08:30

Add to Calendar ▼2014-09-19 08:30:002014-09-19 09:30:00Europe/LondonNanoparticle Flow Cytometry of Individual Extracellular VesiclesExosomes and Single Cell Analysis Summit in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com

Extracellular vesicles (EVs) released from cells have attracted much attention as potential biomarkers and therapeutic targets, but their small size presents a challenge for analysis. Because EVs can originate from many cell types and from different membrane compartments within a cell, EVs from blood or other fluids are intrinsically heterogeneous, and require a multiparameter measurement approach to identify sub-populations that might have physiological relevance. Flow cytometry is perhaps the most widely used multiparameter single particle analysis platform, but commercial instruments lack the sensitivity to detect the vast majority of EVs. We have developed a custom Nanoparticle Flow Cytometer (NFC) and associated methods that can rapidly analyze individual membrane vesicles as small as 70 nm in diameter and resolve particles with less than 100 fluorescent antibodies bound to their surface. I will describe the measurement principles of the NFC, calibration and validation experiments, and present results of EV measurements of cell culture supernatants and blood. This ability to make quantitative, multiparameter measurements of individual EVs will be a key to understanding the roles of these nanoparticles in health and disease.