Sean Bendall,
Assistant Professor,
Stanford University School of Medicine
Sean C. Bendall is an assistant professor in the Department of Pathology at Stanford University, School of Medicine. His research specialty is single-cell proteomic investigations of developing human systems. Sean’s PhD work involved dissecting protein regulators of human pluripotency using embryonic stem cells and mass spectrometry-based proteomics. He then went onto Stanford University where he trained under Professor Garry Nolan, pioneering CyTOF mass cytometry - a next-generation single-cell analysis platform. Sean’s work in mass cytometry analysis has gone on to provide an unparalleled granularity of understanding in multiple facets of hematopoiesis and immunology. His work has been recognized by numerous awards including the Damon Runyon Cancer Research Foundation “Breakthrough Scientist” award and the International Society for the Advancement of Cytometry President’s Award of Excellence.
Hallmarks and Regulatory Coordination Points in Human Development Revealed by Single Cell Trajectory Detection
Thursday, 18 September 2014 at 14:30
Add to Calendar ▼2014-09-18 14:30:002014-09-18 15:30:00Europe/LondonHallmarks and Regulatory Coordination Points in Human Development Revealed by Single Cell Trajectory DetectionExosomes and Single Cell Analysis Summit in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Tissue regeneration is an orchestrated progression of cells from an immature state to a mature one, conventionally represented as distinctive cell subsets landmarked along development. Yet, a continuum of transitional cell states is exists between these discretized stages. We combine the deep phenotyping of single-cell mass cytometry to leverage this continuum by aligning cells of a given lineage onto a unified trajectory that accurately predicts the developmental path de novo. Applied to primary human B-cell lymphopoiesis, the algorithm (termed Wanderlust) constructed trajectories spanning from hematopoietic stem cells through to naïve B cells. Analysis of this trajectory revealed nascent fractions of B-cell progenitors and aligned them with developmentally cued regulatory signaling (IL-7/STAT5) and cellular events (immunoglobulin rearrangement), highlighting developmental checkpoints across which regulatory signals are rewired paralleling changes in cellular state. This study provides the most comprehensive phenotypic analysis of human B lymphopoiesis to date, laying a foundation to apply such methods to other tissue types and “corrupted” developmental processes such as cancer.
Add to Calendar ▼2014-09-18 00:00:002014-09-19 00:00:00Europe/LondonExosomes and Single Cell Analysis SummitExosomes and Single Cell Analysis Summit in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2014-09-18 14:30:002014-09-18 15:30:00Europe/LondonHallmarks and Regulatory Coordination Points in Human Development Revealed by Single Cell Trajectory DetectionExosomes and Single Cell Analysis Summit in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
