Immuno-Affinity Magnetic Beads Based Exosome Isolation MethodFriday, 19 September 2014 at 13:30 Add to Calendar ▼2014-09-19 13:30:002014-09-19 14:30:00Europe/LondonImmuno-Affinity Magnetic Beads Based Exosome Isolation MethodExosomes and Single Cell Analysis Summit in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com In order to isolate exosomes from various body fluids and cell culture supernatants, we have successfully developed ExoCapTM Isolation Kits, which utilizes a magnetic beads based isolation method. ExoCap consists of magnetic particles coupled with antibodies that recognize antigens on the exosome surface, a diluent/ washing solution, and a reagent that releases the captured intact exosomes for analysis. The antibodies against CD9, CD63, CD81, and EpCAM were specifically selected for this kit. ExoCap can easily and rapidly separate exosomes within 30 minutes, without an ultracentrifuge or any special equipment. In exosome rich samples, as little as 100 µL is a sufficient sample volume for the isolation of exosomes that are compatible with various downstream analysis such as western blots RT-qPCR or electron microscopy. ExoCap allows the enrichment of a pure population of specific exosomes based upon target antigen. |