Lidong Qin,
Professor and CPRIT Scholar,
Houston Methodist Research Institute
Dr. Lidong Qin received his Ph.D. degree from Northwestern University and completed postdoctoral training from California Institute of Technology, in 2007 and 2010 respectively. He moved to Houston Methodist Research Institute in 2010 as an Assistant Professor and was promoted to Associated Professor and Full Professor in 2013 and 2016. He is also a CPRIT scholar awarded by the Cancer Research and Prevention Institute of Texas. His current research interest is in microfluidics biotechnology for translational medicine.
Imaging of Cell–Cell Communication in a Vertical Orientation Reveals High-Resolution Structure of Immunological Synapse
Wednesday, 28 September 2016 at 08:30
Add to Calendar ▼2016-09-28 08:30:002016-09-28 09:30:00Europe/LondonImaging of Cell–Cell Communication in a Vertical Orientation Reveals High-Resolution Structure of Immunological SynapseNGS, SCA, SMA and Mass Spec: Research to Diagnostics 2016 in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
The immunological synapse (IS) is one of the most pivotal communication strategies in immune cells. Understanding the molecular basis of the IS provides critical information regarding how immune cells mount an effective immune response. Fluorescence microscopy provides a fundamental tool to study the IS. However, current imaging techniques for studying the IS cannot sufficiently achieve high resolution in real cell–cell conjugates. In this study, we present a new device that allows for high-resolution imaging of the IS with conventional confocal microscopy in a high-throughput manner. Combining micropits and single-cell trap arrays, we have developed a new microfluidic platform that allows visualization of the IS in vertically “stacked” cells. Using this vertical cell pairing (VCP) system, we investigated the dynamics of the inhibitory synapse mediated by an inhibitory receptor, programed death protein-1, and the cytotoxic synapse at the single-cell level. In addition to the technique innovation, we have demonstrated novel biological findings by this VCP device, including novel distribution of F-actin and cytolytic granules at the IS, programed death protein-1 microclusters at the NK IS, and kinetics of cytotoxicity. We propose that this high-throughput, cost-effective, easy-to-use VCP system, along with conventional imaging techniques, can be used to address a number of significant biological questions in a variety of disciplines.
Add to Calendar ▼2016-09-26 00:00:002016-09-28 00:00:00Europe/LondonNGS, SCA, SMA and Mass Spec: Research to Diagnostics 2016NGS, SCA, SMA and Mass Spec: Research to Diagnostics 2016 in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2016-09-28 08:30:002016-09-28 09:30:00Europe/LondonImaging of Cell–Cell Communication in a Vertical Orientation Reveals High-Resolution Structure of Immunological SynapseNGS, SCA, SMA and Mass Spec: Research to Diagnostics 2016 in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
