Alexander Asanov,
Chief Scientific Officer,
TIRF Labs, Inc.
Before founding TIRF Labs in 2004, Dr. Asanov held academic positions at the Institute of Chemical Physics, Russian Academy of Sciences (RAS), the University of Alabama at Birmingham, and Mississippi State University. He received M.S. degree in Biophysics from the Moscow Institute for Physics and Technology, and Ph.D. degree in Chemical Physics from the Institute of Chemical Physics, RAS. His Ph.D. advisor was Nobel Prize laureate academician N. N. Semenov. In the period 2004-2011 Dr. Asanov served as the Principal Investigator on several BAA and SBIR projects sponsored by the U.S. government through the National Institutes of Health and the U.S. Homeland Security Advanced Research Projects Agency (HSARPA).
Total Internal Reflection Fluorescence for Single Cell Analysis at the Level of Single Molecules
Wednesday, 28 September 2016 at 10:00
Add to Calendar ▼2016-09-28 10:00:002016-09-28 11:00:00Europe/LondonTotal Internal Reflection Fluorescence for Single Cell Analysis at the Level of Single MoleculesNGS, SCA, SMA and Mass Spec: Research to Diagnostics 2016 in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Total Internal Reflection Fluorescence (TIRF) has become a method of choice for single molecule detection and is establishing itself as a powerful tool for other areas of lifesciences, including membrane biophysics, real-time microarrays, and molecular diagnostics. Here, we report on a novel application of TIRF for single cell analysis. We combined TIRF with dielectrophoresis and real-time microarrays to perform lysis or electroporation of cells directly at the TIRF surface. Real-time microarrays printed at the TIRF surface allow for the multiplexed analysis of protein and nucleic acid markers obtained from single cells with the limit of detection down to single molecules. In this presentation, we will also describe the requirements for single molecule detection, and will compare prism-, lightguide-, and objective-based TIRF geometries for single molecule studies. This presentation will discuss the advantages and limitations of different TIRF geometries for single cell analysis, ion channel and dynamics of lipid rafts studies, and molecular diagnostics applications.
Add to Calendar ▼2016-09-26 00:00:002016-09-28 00:00:00Europe/LondonNGS, SCA, SMA and Mass Spec: Research to Diagnostics 2016NGS, SCA, SMA and Mass Spec: Research to Diagnostics 2016 in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2016-09-28 10:00:002016-09-28 11:00:00Europe/LondonTotal Internal Reflection Fluorescence for Single Cell Analysis at the Level of Single MoleculesNGS, SCA, SMA and Mass Spec: Research to Diagnostics 2016 in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
