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SELECTBIO Conferences AgriGenomics World Congress

Tamas Dalmay's Biography



Tamas Dalmay, Director/Professor, University of East Anglia

Tamas Dalmay graduated from the Corvinus University, Budapest in 1990 and later obtained his PhD on molecular virology from the Agricultural Biotechnology Centre, Hungary. He spent seven years in David Baulcombe’s lab at the Sainsbury Laboratory (Norwich, UK) as a postdoctoral researcher. During this period he identified several genes required for transgene silencing in Arabidopsis. In 2002 he started his own group at the School of Biological Sciences at the University of East Anglia (Norwich). The last ten years his group investigated various aspects of regulatory small RNAs in fungi, plants and animal/human cells.

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The Impact of Ligation Bias on Plant Small RNA Sequencing

Tuesday, 24 September 2013 at 16:30

Add to Calendar ▼2013-09-24 16:30:002013-09-24 17:30:00Europe/LondonThe Impact of Ligation Bias on Plant Small RNA SequencingAgriGenomics World Congress in John Innes Centre, Norwich, UKJohn Innes Centre, Norwich, UKSELECTBIOenquiries@selectbiosciences.com

RNA ligases used during library generation for small RNA sequencing by NGS prefer certain molecules, which can form stable secondary structures with the adapters. We have developed new adapters called high definition (HD) adapters that contain degenerated nucleotides, which reduce the ligation bias. cDNA libraries of small RNAs were generated from four plant species using either traditional or HD adapters and sequenced by NGS. The impact of the ligation bias on plant small RNA sequencing will be presented at the conference.


Add to Calendar ▼2013-09-24 00:00:002013-09-25 00:00:00Europe/LondonAgriGenomics World CongressAgriGenomics World Congress in John Innes Centre, Norwich, UKJohn Innes Centre, Norwich, UKSELECTBIOenquiries@selectbiosciences.com