The Impact of Ligation Bias on Plant Small RNA SequencingTuesday, 24 September 2013 at 16:30 Add to Calendar ▼2013-09-24 16:30:002013-09-24 17:30:00Europe/LondonThe Impact of Ligation Bias on Plant Small RNA SequencingAgriGenomics World Congress in John Innes Centre, Norwich, UKJohn Innes Centre, Norwich, UKSELECTBIOenquiries@selectbiosciences.com RNA ligases used during library generation for small RNA sequencing by NGS prefer certain molecules, which can form stable secondary structures with the adapters. We have developed new adapters called high definition (HD) adapters that contain degenerated nucleotides, which reduce the ligation bias. cDNA libraries of small RNAs were generated from four plant species using either traditional or HD adapters and sequenced by NGS. The impact of the ligation bias on plant small RNA sequencing will be presented at the conference. |